Novel evaluation method of oxidative stress in pediatric patients with non-alcoholic fatty liver disease

• Project/Area Number: 15K09703
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Osaka Medical College
• Principal Investigator: TAMAI HIROSHI 大阪医科大学, 医学部, 教授 (30179874)
• Co-Investigator(Kenkyū-buntansha): 瀧谷 公隆 大阪医科大学, 医学部, 教授 (80319540)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脂肪性肝疾患 / ビタミンE / 酸化ストレス / ビタミン

Outline of Final Research Achievements

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease, and its incidence is increasing in adults and children. Vitamin E is useful for treatment of NAFLD as an antioxidant therapy, however the mechanism of the therapy is remained to be determined. Previously, we demonstrated that altered hepatic α-tocopherol transfer protein (α-TTP) expression may have a key role in the treatment of vitamin E for methionine-choline deficient (MCD) rats as the experimental model of NAFLD. In the current study, we suggested that afamin, the binding protein of α-tocopherol, may affect α-tocopherol status in the liver and circulation, and have the relationship with the reduced lipid peroxidation.

Academic Significance and Societal Importance of the Research Achievements

脂肪肝と非アルコール性脂肪肝炎との鑑別は、臨床上の有力な鑑別方法はない。しかし非アルコール性脂肪肝炎疑い症例を効率よく抽出することは、検査方法を組み合わせることで可能かもしれない。我々の研究は、早期の非アルコール性脂肪肝炎疑い症例の検出に寄与することが期待される。さらに小児期における生活習慣病の予防に有益な情報を与えることが考えられる。また、非アルコール性脂肪肝疾患における抗酸化療法の新たな戦略を見いだすことが期待される。

Research Products

• [Journal Article] Altered Expression of Retinol Metabolism-Related Genes in an ANIT-Induced Cholestasis Rat Model. (2018)
  • Author(s): Takitani K, Kishi K, Miyazaki H, Koh M, Tamaki H, Inoue A, Tamai H.
  • Journal Title: Int J Mol Sci. Volume: 19 Issue: 11 Pages: 3337-3337
  • DOI: 10.3390/ijms19113337
  • Peer Reviewed / Open Access
• [Journal Article] Microvascular endothelial function in Japanese early adolescents (2017)
  • Author(s): Odanaka Y, Takitani K, Katayama H, Fujiwara H, Kishi K, Ozaki N, Ashida A, Takaya R, Tamai H.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 61 Issue: 3 Pages: 228-232
  • DOI: 10.3164/jcbn.17-58
  • NAID: 130006191923
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Evaluation of uric acid levels, thyroid function, and anthropometric parameters in Japanese children with Down syndrome (2017)
  • Author(s): Niegawa T, Takitani K, Takaya R, Ishiro M, Kuroyanagi Y, Okasora K, Minami Y, Matsuda T, Tamai H.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 61 Issue: 2 Pages: 146-152
  • DOI: 10.3164/jcbn.17-55
  • NAID: 130006038883
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Dehydroepiandrosterone alters vitamin E status and prevents lipid peroxidation in vitamin E-deficient rats (2016)
  • Author(s): Miyazaki H, Takitani K, Koh M, Inoue A, Tamai H.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 58 Issue: 3 Pages: 223-231
  • DOI: 10.3164/jcbn.15-133
  • NAID: 130005149367
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] Dehydroepiandrosterone Alters Retinol Status and Expression of the β-Carotene 15,15′-Monooxygenase and Lecithin:Retinol Acyltransferase Genes (2016)
  • Author(s): Takitani K, Miyazaki H, Koh M, Kishi K, Inoue A, Tamai H.
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 62 Issue: 1 Pages: 12-18
  • DOI: 10.3177/jnsv.62.12
  • NAID: 130005148613
  • ISSN: 0301-4800, 1881-7742
  • Peer Reviewed / Open Access
• [Journal Article] Association of thyroid hormones with obesity and metabolic syndrome in Japanese children (2015)
  • Author(s): Minami Y, Takaya R, Takitani K, Ishiro M, Okasora K, Niegawa T, Tamai H.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 57 Issue: 2 Pages: 121-128
  • DOI: 10.3164/jcbn.15-24
  • NAID: 130005096227
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Altered retinol status and expression of retinol-related proteins in streptozotocin-induced type 1 diabetic model rats (2015)
  • Author(s): Takitani K, Inoue K, Koh M, Miyazaki H, Kishi K, Inoue A, Tamai H.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 56 Issue: 3 Pages: 195-200
  • DOI: 10.3164/jcbn.14-113
  • NAID: 130005068186
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Remarks] 大阪医科大学 小児科
  • URL: http://www.osaka-med.ac.jp/deps/ped/index.html
• [Remarks] 大阪医科大学小児科
  • URL: http://www.osaka-med.ac.jp/deps/ped/index.html