| Project/Area Number |
15K10013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
Sudo Hitomi 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 分子イメージング診断治療研究部, 研究員(任常) (10415416)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUJI Atsushi 放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (60303559)
SUGYO Aya 放射線医学総合研究所, 分子イメージング研究センター, 技術員 (00415415)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 放射線応答遺伝子 / Cre/loxP発現制御システム / Cre/loxPシステム / ルシフェラーゼアッセイ |
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| Outline of Final Research Achievements |
In order to accurately understand the biological effects of radiation, especially the mechanism of radiation-induced cancer, it is important to observe cells individually as “individual cells”, not "group of cells". Therefore, it is necessary a system that distinguishes the presence or absence of radiation exposure within individual living cells and follows up over long period. However, it has not been reported a suitable system at the present time. In this study, we constructed the expression vectors including radiation-responsive sequences inducing the expression of a downstream reporter genes and developed “radiation exposed-cell tracking system”, which allow to visualize irradiated cells individually within living cells
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