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Development of radiation exposed-cell tracking system for imaging within living cells

Research Project

Project/Area Number 15K10013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Radiation science
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology

Principal Investigator

Sudo Hitomi  国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 分子イメージング診断治療研究部, 研究員(任常) (10415416)

Co-Investigator(Renkei-kenkyūsha) TSUJI Atsushi  放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (60303559)
SUGYO Aya  放射線医学総合研究所, 分子イメージング研究センター, 技術員 (00415415)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords放射線応答遺伝子 / Cre/loxP発現制御システム / Cre/loxPシステム / ルシフェラーゼアッセイ
Outline of Final Research Achievements

In order to accurately understand the biological effects of radiation, especially the mechanism of radiation-induced cancer, it is important to observe cells individually as “individual cells”, not "group of cells". Therefore, it is necessary a system that distinguishes the presence or absence of radiation exposure within individual living cells and follows up over long period. However, it has not been reported a suitable system at the present time. In this study, we constructed the expression vectors including radiation-responsive sequences inducing the expression of a downstream reporter genes and developed “radiation exposed-cell tracking system”, which allow to visualize irradiated cells individually within living cells

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2017

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 放射線被ばく細胞のデュアル蛍光レポーターイメージングシステムの構築2017

    • Author(s)
      須藤仁美、須堯綾、相良雅史、辻厚至、東達也
    • Organizer
      日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effects of alpha-emitting meta-211At-astato-benzylguanidine (211At-MABG) on tumor growth suppression in a pheochromocytoma mouse model with histological analysis2017

    • Author(s)
      H. Sudo, A. Sugyo, A. Tsuji, K. Nagatsu, Y. Ohshima, N. S. Ishioka, T. Higashi, K. Yoshinaga
    • Organizer
      Society of Nuclear Medicine and Molecular Imaging Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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