Examination of in vivo performance of human iPS-derived bioartificial liver

Research Project

Project/Area Number	15K10023
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	General surgery
Research Institution	Kansai Medical University (2016-2017) Kyoto University (2015)
Principal Investigator	SHIROUZU Yasumasa 関西医科大学, 医学部, 講師 (20279186)
Co-Investigator(Kenkyū-buntansha)	角昭一郎京都大学, ウイルス・再生医科学研究所, 准教授 (80252906)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	iPS細胞 / バイオ人工肝 / ヒトiPS細胞 / 肝細胞 / 内胚葉
Outline of Final Research Achievements	We have succeeded in the highly efficient differentiation of human pluripotent stem cells to the hepatic progenitor cells under the chemically defined condition, which were able to differentiate to the mature hepatocytes. Moreover, freezing them as dissociated cells made the cell viability after thawing improved substantially. This technique was applied to making bioartificial liver, which had the hepatic progenitor cells in freezed polyvinyl alcohol. The bioartifical liver therefore could have the more viable cells, and the application to the transplantation experiment was made possible.