Establishment of a novel regulating method for allogeneic or xenogeneic antibody-mediated rejection using a humanized mouse
| Project/Area Number |
15K10025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Research Collaborator |
柳川 泉一郎 , 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 異種移植 / 同種移植 / ヒト化マウス / 移植免疫 / 抗体関連型拒絶反応 / 慢性拒絶反応 / HLA抗体 |
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| Outline of Final Research Achievements |
In order to establish a HLA antibody-producing humanized mouse model using human cells, human peripheral blood was cultured in a culture system in which a transduction signal (CD40-CD40 ligand) for activating antibody-producing B cells was transfected, and HLA Antibody-producing humanized mice were born. However, only the target anti-donor- specific HLA antibody production was found to be inclined to inhibit production. Indicating the possibility of being a mouse model capable of verifying the mechanism of donor specific antibody production inhibition. Heterologous antibody-producing humanized mouse models could produce immunodeficient mice lacking heterologous antigens, but since they are not stable, humanized heterologous antibody-producing mouse models transfected with human cells have not been established yet.
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Report
(4 results)
Research Products
(10 results)
