Regeneration of circumferential esophagus with peristalsis and enough length for clinical use, in canine experiments

• Project/Area Number: 15K10119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Doshisha University
• Principal Investigator: HAGIWARA Akeo 同志社大学, 生命医科学部, 教授 (90198648)
• Research Collaborator: TORII Hiroko ; OZAMOTO Yuki ; TSUJIMOTO Hiroyuki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 消化管再生 / 動物実験 / 再生医学 / 食道 / 消化管 / 再生 / 羊膜 / 蠕動運動 / 全周性 / 足場材料 / 消化器 / 血管新生 / 大網 / 血流 / 再生足場 / 腹腔内培養

Outline of Final Research Achievements

Transplantation of mucosal epithelial tissues of the digestive canal is difficult. In the present research hepatic cells were used instead of the epithelial cells of the mucosal cells. The scaffolds where liver cells were disseminated were rolled with the greater omentum into a shape of tube, and then the tube was incubated in the peritoneal cavity of the animal. Microscopically it was confirmed that the blood vessels infiltrated into the scaffolds from the omentum and the liver cells grew successfully in the layers of the scaffolds.

Research Products

• [Journal Article] New polyglycolic acid fabric for the prevention of postoperative pancreatic fistulas Takagi T, Hagiwara A and three others (2018)
  • Author(s): Takagi T, Hagiwara A and three others
  • Journal Title: Asian J Surgery Volume: 41 Pages: 59-64
  • Peer Reviewed / Open Access
• [Journal Article] Sialic level is significantly elevated in IgM enriched protein fraction in sera of cancer patients (2017)
  • Author(s): Torii T, Kanemitsu K, Hagiwara A
  • Journal Title: J Immunoassay Immunochem Volume: 38 Issue: 2 Pages: 127-139
  • DOI: 10.1080/15321819.2016.1224973
  • Peer Reviewed
• [Journal Article] Anti-adhesive effects of a newly developed two-layered gelatin sheet in dogs (2017)
  • Author(s): Torii T, Tsujimoto H, Hagiwara A, et al
  • Journal Title: J Obstet Gynecol Res Volume: 43(8) Issue: 8 Pages: 1317-1325
  • DOI: 10.1111/jog.13358
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The influences of a novel anti-adhesion device, thermally cross-linked gelatin film on peritoneal dissemination of tumor cells (2017)
  • Author(s): Miyamoto H, Hagiwara A, and 6 others
  • Journal Title: J Biomedical Materials Research B Volume: 105 Issue: 6 Pages: 1-9
  • DOI: 10.1002/jbm.b.34017
  • Peer Reviewed / Open Access
• [Journal Article] Physical and Biological Properties of a Novel Anti-adhesion Material Made of Thermally Cross-linked Gelatin Film: Investigation of the Usefulness as Anti-adhesion Material (2017)
  • Author(s): Tsunehito Horii, Hiroyuki Tsujimoto, Hiroe Miyamoto, Koki Yamanaka, Shota Tanaka, Hiroko Torii, Yuki Ozamoto, Hideki Takamori, Eiji Nakamachi, Yoshito Ikada, Akeo Hagiwara
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: - Issue: 2 Pages: 1-8
  • DOI: 10.1002/jbm.b.33880
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] New polyglycolic acid fabric for the prevention of postoperative pancreatic fistulas (2016)
  • Author(s): Toshitaka Takagi, Hiroyuki Tsujimoto, Hiroki Torii, Yuki Ozamoto, Akeo Hagiwara
  • Journal Title: Asian Journal of Surgery Volume: - Issue: 6 Pages: 519-524
  • DOI: 10.1007/s00104-015-0003-2
  • Peer Reviewed / Open Access
• [Presentation] Novel anti-adhesion devices using scaffold material for regenerative medicine (2016)
  • Author(s): Hiroyuki Tsujimoto, Tsunehisa Horii, Hiroe Miyamoto, Hiroko Torii, Yuki Ozamoto, Toshitaka Takagi, Hideki Takamori, Shuko Suzuki, Shinichiro Morita, Yoshito Ikada, Akeo Hagiwara
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 京都国際会議場、京都市、日本
  • Year and Date: 2016-10-23
  • Int'l Joint Research