Comprehensive analyses of DNA methylation for the development of pouchitis after surgery in patients with ulcerative colitis

• Project/Area Number: 15K10136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: Kawamura Mikio 三重大学, 医学系研究科, リサーチアソシエイト (00722589)
• Co-Investigator(Kenkyū-buntansha): 問山 裕二 三重大学, 医学部附属病院, 講師 (00422824) ; 大北 喜基 三重大学, 医学部附属病院, 助教 (20378342) ; 楠 正人 三重大学, 医学系研究科, 教授 (50192026) ; 荒木 俊光 三重大学, 医学系研究科, 准教授 (70343217)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 回腸嚢炎 / 潰瘍性大腸炎 / DNA methylation / micro RNA / サイトカイン

Outline of Final Research Achievements

Comprehensive microRNA and mRNA expression patterns related to pouchitis were analyzed using ileal mucosa at the time of colectomy. Interferon gamma and FK506 binding protein mRNA levels in ileal mucosa could be predictive for the onset of pouchitis after surgery.
Using epigenomic analysis, we found specific micro RNAs to be methylated in rectal mucosal samples from patients with ulcerative colitis associated cancer, and could be used to identify patients with ulcerative colitis at high risk for developing neoplasia.

Research Products

• [Journal Article] A Panel of Methylated MicroRNA Biomarkers for Identifying High-Risk Patients With Ulcerative Colitis-Associated Colorectal Cancer. (2017)
  • Author(s): Toiyama Y, Okugawa Y, Tanaka K, Araki T, Uchida K, Hishida A, Uchino M, Ikeuchi H, Hirota S, Kusunoki M, Boland CR, Goel A.
  • Journal Title: Gastroenterology. Volume: 153 Issue: 6 Pages: 1634-1646
  • DOI: 10.1053/j.gastro.2017.08.037
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 大腸全摘時回腸粘膜におけるTh1,Th2,Th17 cytokine profileと回腸嚢炎発症の関連性 (2016)
  • Author(s): 川村幹雄
  • Organizer: 第102回日本消化器病学会総会
  • Place of Presentation: 京王プラザホテル(東京都・新宿区)
  • Year and Date: 2016-04-21
• [Presentation] 潰瘍性大腸炎術後回腸嚢炎発症と大腸全摘時回腸粘膜中Interferon-γの発現との関連性 (2016)
  • Author(s): 川村幹雄
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: リーガロイヤルホテル大阪(大阪府・大阪市)
  • Year and Date: 2016-04-14