Prognostic value of circulating tumour DNA in patients undergoing curative resection for pancreatic cancer.
Project/Area Number |
15K10184
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Hiroshima University |
Principal Investigator |
Murakami Yoshiaki 広島大学, 医歯薬保健学研究科(医), 准教授 (10263683)
|
Co-Investigator(Kenkyū-buntansha) |
橋本 泰司 広島大学, 学内共同利用施設等, その他 (50423380)
上村 健一郎 広島大学, 医歯薬保健学研究科(医), 講師 (60379873)
|
Research Collaborator |
HADANO Naoto 広島大学, 大学院生
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 膵癌 / バイオマーカー / K-ras変異 / cell free DNA / cell-freeDNA / K-ras遺伝子変異 / 効果予測因子 / Circulating Tumor Cell / Droplet Digital PCR / K-RAS遺伝子変異 / cell-free DNA / Circulating tumor cell / KRAS遺伝子変異 |
Outline of Final Research Achievements |
We used droplet digital polymerase chain reaction to detect rare mutant tumour-derived KRAS genes in plasma cell-free DNA (cfDNA) as ctDNA. Samples were collected from 105 patients who underwent pancreatoduodenectomy for PDAC at a single institution. Overall survival (OS) was analysed according to the presence of ctDNA.Among the 105 cases, ctDNA was detected in 33 (31%) plasma samples. The median OS durations were 13.6 months for patients with ctDNA (ctDNA+) and 27.6 months for patients without ctDNA. Patients who were ctDNA+ had a significantly poorer prognosis with respect to OS (P<0.0001).Our findings suggested that the presence of ctDNA in plasma samples could be an important and powerful predictor of poor survival in patients with PDAC. Accordingly, ctDNA detection might be a promising approach with respect to PDAC treatment.
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Report
(4 results)
Research Products
(7 results)