| Project/Area Number |
15K10191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
美馬 浩介 熊本大学, 医学部附属病院, 非常勤診療医師 (00546559)
中川 茂樹 熊本大学, 医学部附属病院, 医員 (10594872)
岡部 弘尚 熊本大学, 医学部附属病院, 助教 (40573621)
林 洋光 熊本大学, 医学部附属病院, 非常勤診療医師 (80625773)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肝内胆管癌 / EZH2 / VASH1 / 血管新生 / 細胞増殖 / Notch1 / DZNep / 胆管癌 / 細胞周期 / アポトーシス / Angiogenesis |
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| Outline of Final Research Achievements |
Enhancer of zeste homolog 2 (EZH2) is the catalytic subunit of the polycomb repressive complex 2 (PRC2) and regulate tumor malignancy by gene silencing via histone methylation. In this study, we investigate the role of EZH2 in angiogenesis of intrahepatic cholangiocarcinoma (ICC). The influence of EZH2 on tumor angiogenesis and the prognostic significance of EZH2 and VASH1 expression was also examined in ICC cohort by IHC.
By bioinformatical analysis, EZH2 was associated with several angiogenesis gene set in public database. We also find that EZH2 suppress VASH1 expression in vitro assay and IHC study. In IHC analysis. EZH2-high/VASH1-low status was independently associated with poor disease-free survival (P=0.019) and poor overall survival (P=0.0055). The current study demonstrated that high EZH2 expression was associated with activation of tumor angiogenesis, and the EZH2 mediated angiogenesis pathway predicts the prognosis of patients with ICC.
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