| Project/Area Number |
15K10217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
SHIRASAWA Bungo 山口大学, 大学院医学系研究科, 教授 (80444719)
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| Co-Investigator(Kenkyū-buntansha) |
藏澄 宏之 山口大学, 医学部, 特別医学研究員 (50645116)
末廣 晃太郎 山口大学, 医学部附属病院, 助教 (10569312)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 間葉系幹細胞 / 細胞シート / 低酸素プレコンディショニング / 心筋梗塞 |
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| Outline of Final Research Achievements |
We investigated the in vivo therapeutic effect of rabbit bone marrow-derived mesenchymal stem cell sheets (BM-MSC) on a rabbit old myocardial infarcted area following autologous transplantation. Functions of left ventricular and myocardial infarcted area were compared among control group, transplantation group of BM-MSC following normoxic culture condition, and transplantation group of BM-MSC following hypoxic culture condition. Functions of left ventricular was evaluated left ventricular ejection fraction and left ventricular fractioning shortening, and myocardial infarcted area was evaluated Masson's trichrome-staining. Compared with control, transplantation of BM-MSC following normoxic condition significantly enhanced functions of left ventricular and reduced myocardial infarcted area. Furthermore, compared with normoxic condition, transplantation of BM-MSC following hypoxic condition significantly enhanced functions of left ventricular and reduced myocardial infarcted area.
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