Useful Imaging to detect Macrophage Localization in the Media and Adventitia of the Abdominal Aortic Aneurysm Wall using Ex Vivo Superparamagnetic Iron Oxide-Enhanced Magnetic Resonance Imaging
| Project/Area Number |
15K10232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
Shimizu Takuya 東北大学, 医学系研究科, 大学院非常勤講師 (70579755)
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| Co-Investigator(Kenkyū-buntansha) |
後藤 均 東北大学, 医学系研究科, 准教授 (00400333)
大田 英揮 東北大学, 医学系研究科, 准教授 (40586905)
中村 保宏 東北大学, 医学系研究科, 大学院非常勤講師 (80396499)
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| Research Collaborator |
UMETSU Michihisa
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | SPIO MRI / 腹部大動脈瘤 / マクロファージの局在診断 / SPIO MRI / 組織画像診断 / MRI |
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| Outline of Final Research Achievements |
Macrophages in the aneurysmal wall play an important role in the pathogenesis of abdominal aortic aneurysms (AAAs). superparamagnetic iron oxide (SPIO) is a macrophage-specific contrast agent that result in negative enhancement on T2*-weighted imaging. We attempted to evaluate macrophage localization in the media and adventitia of AAA wall using SPIO-enhanced MRI. Ex vivo MRI was performed for the specimens in SPIO group. There was significant positive correlation between berlin blue-stained macrophages and berlin blue positive area in SPIO group with macrophages. We divided the media and adventitia in each specimen and compared the ROI of T2*-weighted SPIO-enhanced MRI with berlin blue-stained area. We found significant correlations in 5 of 7 cases with macrophages. We detected that ex vivo T2*-weighted SPIO-enhanced MR findings visualize a macrophage localization in the media and adventitia. This imaging technique would also visualize the macrophage distribution in in-vivo MRI.
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| Academic Significance and Societal Importance of the Research Achievements |
腹部大動脈瘤が拡大する要因はいくつか報告されているものの、十分には解明されていない。瘤壁内のマクロファージが瘤形成に強く関わっているという組織学的な報告があるが、組織を取らずとも、瘤壁内のマクロファージの局在を示す方法＝可視化ができれば、将来大きくなりやすい瘤なのか、破裂しやすい瘤なのか、新たな指標に繋がる可能性がある。その第一段階として、採取した瘤壁のMRIと組織標本のマクロファージの局在に相関関係があることを明らかにした。今後生体内へ応用することで、新たな指標に繋がる可能性がある。
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Report
(5 results)
Research Products
(1 results)
