Development of a new vascular regenerative therapy for severe limb ischemia by the transplantation of human iPS cell-derived vascular cells
| Project/Area Number |
15K10241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
SAKATA RYUZO 京都大学, 医学研究科, 名誉教授 (20325781)
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| Co-Investigator(Kenkyū-buntansha) |
池田 義 京都大学, 医学研究科, 准教授 (40281092)
山崎 和裕 京都大学, 医学研究科, 講師 (50464227)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA JUN 京都大学, その他部局等, 教授 (50335288)
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| Research Collaborator |
MASUMOTO HIDETOSHI 京都大学, 大学院医学研究科, 特定助教 (70645754)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 末梢動脈外科学 / iPS細胞 / 血管新生 / 血管内皮細胞 / 抹消動脈外科学 |
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| Outline of Final Research Achievements |
The transplantation of human iPS cell-derived endothelial cells and vascular mural cells with gelatin sponges revealed a significant improvement of blood flow and a higher vascular density in the ischemic lindlimb. Ku-80 positive human cells are observed in the newly-formed vasculature indicating the contribution of transplanted human cells toward the vascular regeneration of the host. Furthermore, the transplantation induced the angiogenesis mediated by the up-regulation of paracrine factors. There results would serve as a basis toward the development of a new vascular regenerative therapy for a severe limb ischemia in the future.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Safety and efficacy of sustained release of basic fibroblast growth factor using gelatin hydrogel in patients with critical limb ischemia.2016
Author(s)
Kumagai M, Marui A, Tabata Y, Takeda T, Yamamoto M, Yonezawa A, Tanaka S, Yanagi S, Ito-Ihara T, Ikeda T, Murayama T, Teramukai S, Katsura T, Matsubara K, Kawakami K, Yokode M, Shimizu A, Sakata R.
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Journal Title
Heart Vessels.
Volume: 未定
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Safety and efficacy of sustained release of basic fibroblast growth factor using gelatin hydrogel in patients with critical limb ischemia.2015
Author(s)
Kumagai M, Marui A, Tabata Y, Takeda T, Yamamoto M, Yonezawa A, Tanaka S, Yanagi S, Ito-Ihara T, Ikeda T, Murayama T, Teramukai S, Katsura T, Matsubara K, Kawakami K, Yokode M, Shimizu A, Sakata R.
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Journal Title
Heart and Vessels
Volume: 30
Issue: 5
Pages: 713-721
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.2015
Author(s)
Matsuo T, Masumoto H, Tajima S, Ikuno T, Katayama S, Minakata K, Ikeda T, Yamamizu K, Tabata Y, Sakata R, Yamashita JK.
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Journal Title
Scientific Reports
Volume: 5:16842
Issue: 1
Pages: 1-14
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Impact of geometry and cell density on structural and functional maturation in large-format mesh engineered cardiac tissues.2016
Author(s)
Nakane T, Masumoto H, J P. Tinney, F Yuan, W J. Kowalski, F Ye, Minakata K, Ikeda T, Sakata R, Yamashita J.K, B B.Keller.
Organizer
AHA American Heart Association
Place of Presentation
New Orleans, USA
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] Sequential treatment with sustained-release of basic fibroblast growth factor followed by transplantation of human induced pluripotent stem cell-derived cardiac tissue improved left ventricular function in a rat chronic myocardial infarction model.2016
Author(s)
Zipeng Li, Masumoto H, Minakata K, Kawatou M, Takimoto S, Hirao S, Ikeda T, Tabata Y, Yamashita J.K, Sakata R.
Organizer
AHA American Heart Association
Place of Presentation
New Orleans, USA
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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