Development of an anti-rupture drug treatment method using a novel animal model of easily rupturable large cerebral aneurysm and human clinical specimen
| Project/Area Number |
15K10287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
森脇 拓也 北海道大学, 医学(系)研究科(研究院), 客員研究員 (30597464)
穂刈 正昭 北海道大学, 医学研究院, 客員研究員 (30622807)
鐙谷 武雄 北海道大学, 大学病院, 助教 (80270726)
数又 研 北海道大学, 大学病院, 講師 (60634144)
寳金 清博 北海道大学, 大学病院, 教授 (90229146)
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| Research Collaborator |
SHIMODA Yusuke 北海道大学, 医学研究院, 客員研究員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脳動脈瘤 / 脳動脈瘤破裂 / 大型脳動脈瘤 / 動物モデル / 抗血小板剤 / ラット / くも膜下出血 / 血行力学的負荷 / 内皮 / ニトロ化合物 / 高血圧 |
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| Outline of Final Research Achievements |
In order to elucidate the rupture mechanism of the cerebral aneurysm, we first aimed to establish an animal model of easily rupturable large cerebral aneurysm. Hypertension was induced in male SD rats by ligation of the bilateral renal artery dorsal branch and high salinity, hemodynamic stress is given by left common carotid artery ligation, and the nitro compound was intraperitoneally administered as a stable fumaric acid compound every week. The dose of nitro compound was changed variously, aneurysm developed in about 80% of individuals at 1400 mg / kg, and large aneurysm was induced in 22%.
Furthermore, in order to raise the induction rate of large aneurysms, ovaries were removed in female rats simulating human postmenopausal women, in addition, the contralateral external carotid artery was ligated to enhance the hemodynamic stress. And we started experiments with medication by aspirin and cilostazol.
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Report
(4 results)
Research Products
(6 results)
