Investigation of MDSC-specific marker as prognostic factor and therapeutic target of patients with advanced renal cell carcinoma

• Project/Area Number: 15K10574
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Yamagata University
• Principal Investigator: Kato Tomoyuki 山形大学, 医学部, 准教授 (40396560)
• Co-Investigator(Kenkyū-buntansha): 武田 裕司 山形大学, 医学部, 助教 (90302299)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 腎細胞癌 / MDSC / GPI-80

Outline of Final Research Achievements

The patterns of myeloid-derived suppressor cells (MDSC) in peripheral blood of patients with metastatic renal cell carcinoma (mRCC) were studied and useful prognostic factors were examined. Myeloid cell antigens on peripheral blood neutrophilic cells and monocytic cells in mRCC patients were measured by flow cytometry. A comprehensive RNA expression in polymorphonuclear leukocytes (PMN) was also analyzed. As a result, the MFI (average fluorescence intensity) or CV (coefficient variation) of CD16, GPI-80, and LAP-1 on neutrophilic cells or monocytic cells were increased. It was indicate that the diversity of the myeloid cell population is increased. In addition, mRNA expression of PMNs was different between healthy volunteers and mRCC patients. MFI of GPI-80 on monocytic cells was associated with the clinical outcome and considered to be a useful prognostic marker of mRCC.

Research Products

• [Journal Article] The pattern of GPI-80 expression is a useful marker for unusual myeloid maturation in peripheral blood. (2016)
  • Author(s): Takeda Y, Kato T, Ito H, Kurota Y, Yamagishi A, Sakurai T, Araki A, Nara H, Tsuchiya N, Asao H
  • Journal Title: Clinical and Experimental Immunology Volume: 186 Issue: 3 Pages: 373-386
  • DOI: 10.1111/cei.12859
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 転移性腎細胞癌患者予後因子としての末梢血中GPI-80発現 (2018)
  • Author(s): 加藤智幸、武田裕司、伊藤裕美、黒田悠太、奈良英利、櫻井俊彦、西田隼人、内藤整、川添久、荒木明美、一柳統、浅尾裕信、土谷順彦
  • Organizer: 第106回日本泌尿器科学会総会
• [Presentation] Pattern of glycosylphosphatidylinositol-anchored 80 kD protein (GPI-80) expression in peripheral blood is a useful marker for myeloid-derived suppressor cells (MDSCs) in metastatic renal cell cancer (mRCC) patients (2017)
  • Author(s): Tomoyuki Kato, Yuji Takeda, Yuta Kurota, Hidetoshi Nara, Akemi Araki, Norihiko Tsuchiya, Hironobu Asao
  • Organizer: Genitourinary Cancer Symposium
  • Place of Presentation: オーランド アメリカ合衆国
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] 腎細胞癌患者におけるMDSCマーカーとしての末梢血中GPI-80発現. (2017)
  • Author(s): 1）加藤 智幸, 武田 裕司, 伊藤 裕美, 黒田 悠太, 山岸 敦史, 櫻井 俊彦, 内藤 整, 奈良 英利, 荒木 明美, 浅尾 裕信, 土谷 順彦
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] 転移性腎癌患者末梢血におけるMDSC誘導の指標としてのGP-80発現パターンは新たなMDSC指標探索の基準となる (2017)
  • Author(s): 2）加藤 智幸, 武田 裕司, 伊藤 裕美, 黒田 悠太, 八木 真由, 櫻井 俊彦, 内藤 整, 川添 久, 一柳 統, 土谷 順彦, 浅尾 裕信
  • Organizer: 第76回日本癌学会学術集会
• [Presentation] 転移性腎細胞癌患者における末梢血中GPI-80発現は予後因子となりうる (2017)
  • Author(s): 3）加藤 智幸, 武田 裕司, 伊藤 裕美, 黒田 悠太, 奈良 英利, 櫻井 俊彦, 西田 隼人, 内藤 整, 川添 久, 荒木 明美, 一柳 統, 浅尾 裕信, 土谷 順彦
  • Organizer: 第55回日本癌治療学会学術集会
• [Presentation] 転移性腎癌患者末梢血におけるGPI-80発現パターンはMDSC誘導の指標となる (2016)
  • Author(s): 加藤智幸, 武田裕司, 黒田悠太, 土谷順彦, 浅尾裕信
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市 パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] 腎癌細胞におけるGPI-80の発現とその機能 (2016)
  • Author(s): 黒田悠太, 加藤智幸, 武田裕司, 伊藤裕美, 工藤千恵, 浅尾裕信, 土谷順彦
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市 パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] Divergence of GPI-80 expression in myeloid cells suggests the appearance of myeloid-derived suppressor cells in renal cancer patients. (2015)
  • Author(s): Kato T, Takeda Y, Sakurai T, Ito H, Kawazoe H, Asao H
  • Organizer: 第74回日本癌学会
  • Place of Presentation: 名古屋国際会議場（愛知県 名古屋市 熱田区）
  • Year and Date: 2015-10-08
• [Presentation] Inflammatory cytokines induce the increasing of GPI-80-expression variance during neutrophil differentiation. (2015)
  • Author(s): Takeda Y, Kato T, Sakurai T, Ito H, Kawazoe H, Asao H
  • Organizer: 第74回日本癌学会
  • Place of Presentation: 名古屋国際会議場（愛知県 名古屋市 熱田区）
  • Year and Date: 2015-10-08