| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
This study aimed to assess the utility of microRNAs (miRNAs) as new biomarkers for diagnosis of spermatogenic disorder and clinical or basic research of andrology.
We found the miRNAs showing altered expression specifically in patients with impaired spermatogenesis based on expression profiles of in seminal plasma. Finally, we generated an expression panel with 8 miRNAs related to impaired spermatogenesis through comprehensive microarray analysis of candidate miRNAs.
Determination of miRNA expression levels in seminal plasma with real-time PCR revealed that miR-891a-5p was significantly decreased in the patients with obstructive azoospermia, and miR-1229-3p was significantly increased in the patients with germ cell maturation arrest.
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