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Expression profiling of microRNAs in seminal plasma as a new tool for diagnosis of spermatogenic disorder

Research Project

Project/Area Number 15K10652
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

Yoshiike Miki  聖マリアンナ医科大学, 医学部, 研究技術員 (60398964)

Co-Investigator(Kenkyū-buntansha) 岩本 晃明  国際医療福祉大学, 大学病院, 教授 (60046117)
Research Collaborator Kosaka Nobuyoshi  
Nozawa Shiari  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords造精機能障害 / マイクロRNA / 精漿 / 男性不妊症 / microRNA / miro RNA / 診断マーカー
Outline of Final Research Achievements

This study aimed to assess the utility of microRNAs (miRNAs) as new biomarkers for diagnosis of spermatogenic disorder and clinical or basic research of andrology.
We found the miRNAs showing altered expression specifically in patients with impaired spermatogenesis based on expression profiles of in seminal plasma. Finally, we generated an expression panel with 8 miRNAs related to impaired spermatogenesis through comprehensive microarray analysis of candidate miRNAs.
Determination of miRNA expression levels in seminal plasma with real-time PCR revealed that miR-891a-5p was significantly decreased in the patients with obstructive azoospermia, and miR-1229-3p was significantly increased in the patients with germ cell maturation arrest.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2018

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results)

  • [Journal Article] Association of TUSC1 and DPF3 gene polymorphisms with male infertility2018

    • Author(s)
      Sato Youichi、Hasegawa Chise、Tajima Atsushi、Nozawa Shiari、Yoshiike Miki、Koh Eitetsue、Kanaya Jiro、Namiki Mikio、Matsumiya Kiyomi、Tsujimura Akira、Komatsu Kiyoshi、Itoh Naoki、Eguchi Jiro、Yamauchi Aiko、Iwamoto Teruaki
    • Journal Title

      Journal of Assisted Reproduction and Genetics

      Volume: 35 Issue: 2 Pages: 257-263

    • DOI

      10.1007/s10815-017-1052-x

    • NAID

      120006768430

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome-wide association study identifies ERBB4 on 2q34 as a novel locus associated with sperm motility in Japanese men2018

    • Author(s)
      Sato Y, Tajima A, Sato T, Nozawa S, Yoshiike M, Imoto I, Yamauchi A, Iwamoto T
    • Journal Title

      J Med Genet

      Volume: - Issue: 6 Pages: 415-21

    • DOI

      10.1136/jmedgenet-2017-104991

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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