Expression profiling of microRNAs in seminal plasma as a new tool for diagnosis of spermatogenic disorder
Project/Area Number |
15K10652
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Urology
|
Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
Yoshiike Miki 聖マリアンナ医科大学, 医学部, 研究技術員 (60398964)
|
Co-Investigator(Kenkyū-buntansha) |
岩本 晃明 国際医療福祉大学, 大学病院, 教授 (60046117)
|
Research Collaborator |
Kosaka Nobuyoshi
Nozawa Shiari
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 造精機能障害 / マイクロRNA / 精漿 / 男性不妊症 / microRNA / miro RNA / 診断マーカー |
Outline of Final Research Achievements |
This study aimed to assess the utility of microRNAs (miRNAs) as new biomarkers for diagnosis of spermatogenic disorder and clinical or basic research of andrology. We found the miRNAs showing altered expression specifically in patients with impaired spermatogenesis based on expression profiles of in seminal plasma. Finally, we generated an expression panel with 8 miRNAs related to impaired spermatogenesis through comprehensive microarray analysis of candidate miRNAs. Determination of miRNA expression levels in seminal plasma with real-time PCR revealed that miR-891a-5p was significantly decreased in the patients with obstructive azoospermia, and miR-1229-3p was significantly increased in the patients with germ cell maturation arrest.
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Report
(4 results)
Research Products
(2 results)
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[Journal Article] Association of TUSC1 and DPF3 gene polymorphisms with male infertility2018
Author(s)
Sato Youichi、Hasegawa Chise、Tajima Atsushi、Nozawa Shiari、Yoshiike Miki、Koh Eitetsue、Kanaya Jiro、Namiki Mikio、Matsumiya Kiyomi、Tsujimura Akira、Komatsu Kiyoshi、Itoh Naoki、Eguchi Jiro、Yamauchi Aiko、Iwamoto Teruaki
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Journal Title
Journal of Assisted Reproduction and Genetics
Volume: 35
Issue: 2
Pages: 257-263
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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