Identification of labor-onset related mRNA/microRNA and its clinical significance

• Project/Area Number: 15K10677
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: MIURA Shoko 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (00404301)
• Co-Investigator(Kenkyū-buntansha): 三浦 清徳 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (00363490)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Labor / microRNA / plasma / placenta / prediction / 陣痛 / mRNA / 母体血漿 / 陣痛発来 / 分子マーカー / 産婦人科 / 胎盤 / 妊娠経過 / 基準値

Outline of Final Research Achievements

Our study confirmed that C19MC microRNAs express from paternal allele. In addition, our data suggested that circulating level of cell-free miR-518b in maternal plasma is associated with the uterine stimulation. The circulating levels of cell-free C19MC microRNAs in maternal plasma increase as progress of pregnancy, and we could determined their reference values of C19MC microRNA in maternal plasma. Our data showed the possibility that the measurement of circulating C19MC microRNAs in maternal plasma makes it possible to predict the timing of labor-onset and to clarify the pathophysiology of labor-onset.

Research Products

• [Journal Article] Expression levels of C19MC and C14MC microRNAs in complete hydatidiform moles and ovarian mature cystic teratomas (2018)
  • Author(s): Miura K, Hasegawa Y, Yamada M, Higashijima A, Miura S, Kaneuchi M, and Masuzaki H
  • Journal Title: European Journal of Gynaecological Oncology Volume: 39
  • Peer Reviewed
• [Journal Article] Reference values for circulating pregnancy-associated microRNAs in maternal plasma and their clinical usefulness in uncomplicated pregnancy and hypertensive disorder of pregnancy (2018)
  • Author(s): Murakami Y, Miura K, Sato S, Higashijima A, Hasegawa Y, Miura S, Yoshiura KI, Masuzaki H
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 印刷中 Issue: 5 Pages: 840-851
  • DOI: 10.1111/jog.13610
  • Peer Reviewed
• [Journal Article] Circulating levels of pregnancy-associated, placenta-specific microRNAs in pregnant women with placental abruption (2017)
  • Author(s): Miura K, Higashijima A, Murakami Y, Fuchi N, Tsukamoto O, Abe S, Hasegawa Y, Miura S, Masuzaki H
  • Journal Title: Reproductive Science Volume: 24 Pages: 148-155
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A significant association between rs8067378 at 17q12 and invasive cervical cancer originally identified by a genome-wide association study in Han Chinese is replicated in a Japanese population (2017)
  • Author(s): Miura K, Mishima H, Yasunami M, Kaneuchi M, Kitajima M, Abe, S, Fuchi N, Miura S, Yoshiura KI, Masuzaki H
  • Journal Title: Journal of Human Genetics Volume: 印刷中
  • NAID: 40020938176
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A significant association between rs8067378 at 17q12 and invasive cervical cancer originally identified by a genome-wide association study in Han Chinese is replicated in a Japanese population. (2016)
  • Author(s): Miura K, Mishima H, Yasunami M, Kaneuchi M, Kitajima M, Abe S, Higashijima A, Fuchi N, Miura S, Yoshiura KI, Masuzaki H.
  • Journal Title: J Hum Genet. Volume: 61(9) Issue: 9 Pages: 793-796
  • DOI: 10.1038/jhg.2016.50
  • NAID: 40020938176
  • Peer Reviewed
• [Journal Article] Increased levels of cell-free miR-517a and decreased levels of cell-free miR-518b in maternal plasma samples from placenta previa pregnancies at 32 weeks gestation (2015)
  • Author(s): Hasegawa Y, Miura K, Higashijima A, Abe S, Miura S, Yoshiura KI, Masuzaki H
  • Journal Title: Reproductive Sciences Volume: 22 Pages: 1569-1576
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Circulating levels of C19MC-cluster microRNAs in pregnant women with severe pre-eclampsia (2015)
  • Author(s): Miura K, Higashijima A, Murakami Y, Tsukamoto O, Hasegawa Y, Abe S, Naoki Fuchi N, Miura S, Kaneuchi M, Masuzaki H
  • Journal Title: The Journal of Obstetrics and Gynaecology Research Volume: 41 Pages: 1526-1532
  • Peer Reviewed
• [Journal Article] Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy (2015)
  • Author(s): Miura K, Higashijima A, Mishima H, Miura S, Kitajima M, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Fertility and Sterility Volume: 103 Pages: 1202-1208
  • Peer Reviewed
• [Journal Article] Circulating levels of maternal plasma cell-free miR-21 are associated with maternal body mass index and neonatal birth weight (2015)
  • Author(s): Miura K, Higashijima A, Hasegawa Y, Abe S, Miura S, Fuchi N, Murakami Y, Kinoshita A, Yoshida A, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Prenatal Diagnosis Volume: 35 Pages: 509-511
  • Peer Reviewed
• [Journal Article] Effect of labor on plasma concentrations and postpartum clearance of cell-free, pregnancy-associated, placenta-specific microRNAs (2015)
  • Author(s): Morisaki S, Miura K, Higashijima A, Abe S, Miura S, Hasegawa Y, Yoshida A, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Prenatal Diagnosis Volume: 35 Pages: 44-50
  • Peer Reviewed
• [Presentation] 母体血を用いた胎児染色体検査で13トリソミー偽陽性であった1例 (2017)
  • Author(s): 三浦清徳、尾崎 守、長谷川ゆり、三浦生子、増崎英明
  • Organizer: 第25回日本胎盤学会学術集会
• [Presentation] Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy (2015)
  • Author(s): Kiyonori Miura, Ai Higashijima , Yuri Hasegawa, Shoko Miura, Atsushi Yoshida, Masanori Kaneuchi, and Hideaki Masuzaki
  • Organizer: The 19th International Conference on Prenatal Diagnosis and Therapy
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Remarks] 長崎大学医学部産婦人科
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/
• [Remarks] 長崎大学病院産婦人科
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/