Development of a pathophysiological evaluation method for hypertensive disorders of pregnancy by simultaneous quantitation analysis of multiple disease biomarkers.

• Project/Area Number: 15K10686
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Juntendo University
• Principal Investigator: YANAGIDA Mitsuaki 順天堂大学, 医学(系)研究科(研究院), 准教授 (80365569)
• Co-Investigator(Kenkyū-buntansha): 荒木 慶彦 順天堂大学, 医学(系)研究科(研究院), 先任准教授 (70250933)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 妊娠高血圧症候群 / 疾患バイオマーカー / ペプチド / 質量分析 / マルチプルリアクションモニタリング

Outline of Final Research Achievements

Hypertensive disorders of pregnancy (HDP) is a syndrome characterized by high blood pressure that develops after 20-week in pregnancy. The pathogenesis of HDP is not fully elucidated and early diagnosis is expected to be developed. For this purpose, we have focused on the native peptides in the serum of patients with HDP and explored disease related peptides in the patients’ serum. In this study, we established the quantitation method for these peptides using mass spectrometry with high sensitivity, which was multiple reaction monitoring. We measured the candidate biomarker peptides in the serum of patients with HDP using this system, and evaluated whether these peptides were validated as diagnostic markers of HDP.

Research Products

• [Journal Article] Quantitative measurement of a candidate serum biomarker peptide derived from α2-HS-glycoprotein, and a preliminary trial of multidimensional peptide analysis in females with pregnancy-induced hypertension. (2018)
  • Author(s): Hamamura K, Yanagida M, Ishikawa H, Banzai M, Yoshitake H, Nonaka D, Tanaka K, Sakuraba M, Miyakuni Y, Takamori K, Nojima M, Yoshida K, Fujiwara H, Takeda S, Araki Y.
  • Journal Title: Ann Clin Biochem Volume: 55 Issue: 2 Pages: 287-295
  • DOI: 10.1177/0004563217717748
  • Peer Reviewed
• [Journal Article] Simple quantitation for potential serum disease biomarker peptides, primarily identified by a peptidomics approach in the serum with hypertensive disorders of pregnancy. (2016)
  • Author(s): Hamamura K, Nonaka D, Ishikawa H, Banzai M, Yanagida M, Nojima M, Yoshida K, Lee LJ, Tanaka K, Takamori K, Takeda S, Araki Y
  • Journal Title: Ann Clin Biochem Volume: 53 Issue: 1 Pages: 85-96
  • DOI: 10.1177/0004563215583697
  • Peer Reviewed
• [Presentation] 妊娠高血圧症候群で特異的に変動する血中ペプチド測定系の確立 (2017)
  • Author(s): 柳田 光昭、濱村 憲佑、三浦 正子、高森 建二、荒木 慶彦
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）
• [Presentation] 妊娠高血圧症候群で特異的に変動するペプチドの診断利用を指向した評価系の確立 (2016)
  • Author(s): 柳田光昭、濵村憲佑、三浦正子、野中大輔、田中憲次、髙森建二、荒木慶彦
  • Organizer: 日本生化学会年会
  • Place of Presentation: 仙台国際センター（宮城県）
  • Year and Date: 2016-09-25