| Project/Area Number |
15K10687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
牧野 真太郎 順天堂大学, 医学部, 准教授 (70570894)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKAMIZU Akiyoshi 筑波大学, 大学院・生命科学研究科 生命環境学群 生物資源学類, 教授 (60199172)
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| Research Collaborator |
SATO Anna 順天堂大学, 医学研究科, 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク質アルギニン-N-メチル基転移酵素 / 遺伝子欠損マウス / 絨毛細胞特異的ノックアウト / 妊娠高血圧症候群 / PRMT / placenta / mice / アルギニン / ADMA / タンパク質メチル基転移酵素 / 細胞特異的ノックアウト |
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| Outline of Final Research Achievements |
Expression levels and the localization of PRMT1 mRNA and protein in placentas were investigated. In addition, the levels of methylarginines of placental proteins were quantified using liquid chromatography-tandem mass spectrometry.
PRMT1 mRNA and its protein were expressed at highest levels in mid-gestation stages, and their expression showed stepwise decrease in the late gestation. At E9, PRMT1 was observed in several different trophoblast cell (TC) subtypes. Furthermore, PRMT1 was mainly expressed in the labyrinth zone of TCs at E13. Finally, total methylarginines of proteins were significantly reduced in late gestation of placentas compared with mid-gestation stages. We found developmental changes in the placental expression of PRMT1 and in protein arginine methylation status during pregnancy. These findings provide fundamental information regarding placental PRMT1-mediated arginine methylation during the development.
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