Analyses of morphology and function for tricellular junction in human nasal epithelial cells

• Project/Area Number: 15K10788
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Sapporo Medical University
• Principal Investigator: Okuni Tsuyoshi 札幌医科大学, 医学部, 助教 (40464490)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: human nasal epithelium / epithelial barrier / tricellulin / LSR / Capcisin / ヒト正常鼻粘膜上皮細胞 / ASPP2 / PAR3 / バリア機能 / claudins / claudin / HDAC / ヒト鼻粘膜上皮 / 自然免疫 / タイト結合

Outline of Final Research Achievements

In the present study, we investigated the roles of epithelial barrier at tricellular contact of human nasal epithelium by analyses of morphology and function for tricellular junctions. In normal human nasal mucosa tissues, tricellulin and LSR regulated the tricellulin and tricellular contact, expressed at the apicalmost of nasal epithelium.In PCR analysis, not only tricellulin and LSR but also ASPP2 family ASPP1 and ASPP2 interacted with LSR, and PAR3 bind ASPP2 were observed in normal human nasal mucosa tissues.Knockdown of tricellulin or LSR by the siRNAs decrease the epithelial barrier function.When the nasal epithelial cells were treated with Capcisin ,Capcisin induced the localization change of LSR from bicellular region to tricellualr region. Taken together, tricellular tight junction molecules tricellulin and LSR formed tricellular junctions may play crucial roles of human nasal epithelial barrier via the interactions with various tight junction-related molecules.

Research Products

• [Journal Article] The role of transcriptional factor p63 in regulation of epithelial barrier and ciliogenesis of human nasal epithelial cells (2017)
  • Author(s): Kaneko Yakuto、Kohno Takayuki、Kakuki Takuya、Takano Ken-ichi、Ogasawara Noriko、Miyata Ryo、Kikuchi Shin、Konno Takumi、Ohkuni Tsuyoshi、Yajima Ryoto、Kakiuchi Akito、Yokota Shin-ichi、Himi Tetsuo、Kojima Takashi
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 10935-10935
  • DOI: 10.1038/s41598-017-11481-w
  • Peer Reviewed / Open Access
• [Journal Article] Accessory parotid gland tumors: A seres of 4 cases. (2016)
  • Author(s): Kakuki T, Takano K, Kurose M, Kondo A, Okuni T, Ogasawara N, Himi T
  • Journal Title: Ear Nose Throat Volume: 95 Pages: 35-38
  • Peer Reviewed
• [Journal Article] Carcinoma ex pleomorphic adenoma of the parotid gland: a multiple-institutional retrospective analysis in the Northern Japan Head and Neck Cancer Society. (2016)
  • Author(s): Suzuki M, Matsuzuka T, Sajio S, Takahara M, Harabuchi Y, Okuni T, Himi T, Kakizaki T, Fukuda S, Yamada K, Nagahashi T, Abe T, Shinkawa H, Katagiri K, Sato H, Fukui N, Ishikawa K, Suzuki T, Kobayashi T, Saito D, Sajio S, Tateda M, Hashimoto S, Ishida A, Kakehata S, Suzuki O, Hashimoto Y, Omori K
  • Journal Title: Acta Otolaryngol. Volume: 136 Pages: 1154-1158
  • Peer Reviewed
• [Journal Article] A Case Study of Anisakiasis in the Palatine Tonsils. (2016)
  • Author(s): Takano K, Okuni T, Murayama K, Himi T
  • Journal Title: Adv Otorhinolaryngol. Volume: 77 Pages: 125-127
  • DOI: 10.1159/000441903
  • ISBN: 9783318056501, 9783318056518
  • Peer Reviewed
• [Journal Article] Radiological Assessment of the Anatomy of Frontal Recess Cells and the Anterior Ethimoidal Artery. (2016)
  • Author(s): Okuni T, Takano K, Nomura K, Yamashita K, Abe A, Ito F, Murayama K, Shirasaki H, HImi T
  • Journal Title: Adv Otorhinolaryngol. Volume: 77 Pages: 46-51
  • DOI: 10.1159/000441873
  • ISBN: 9783318056501, 9783318056518
  • Peer Reviewed
• [Journal Article] Poly(I:C) induced microRNA-146a regulates epithelial barrier and secretion of proinflammatory cytokines in human nasal epithelial cells. (2015)
  • Author(s): Miyata R, Kakuki T, Nomura K, Ohkuni T, Ogasawara N, Takano K, Konno T, Kohno T, Sawada N, Himi T, Kojima T.
  • Journal Title: Eur J Pharmacol. Volume: 761 Pages: 375-382
  • DOI: 10.1016/j.ejphar.2015.04.031
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ヒト鼻粘膜上皮バリアにおけるp63の役割 (2017)
  • Author(s): 小島隆、金子躍人、金野匠、角木拓也、高野賢一、菊池真、矢島諒人、大国毅、 氷見徹夫、幸野貴之
  • Organizer: 生命科学系学会合同年次大会
• [Presentation] 当科における鼻副鼻腔乳頭腫症例の検討 (2016)
  • Author(s): 大國 毅
  • Organizer: 第55回日本鼻科学会
  • Place of Presentation: 栃木県総合文化センター（栃木県宇都宮市）
  • Year and Date: 2016-10-13
• [Presentation] 入院治療を要した特発性鼻出血症例の検討 (2016)
  • Author(s): 大國 毅
  • Organizer: 第78回耳鼻咽喉科臨床学会
  • Place of Presentation: 城山観光ホテル（鹿児島県鹿児島市）
  • Year and Date: 2016-06-23
• [Presentation] miRNA-146aはヒト鼻粘膜バリアを調節する (2015)
  • Author(s): 宮田遼
  • Organizer: 日本耳鼻咽喉科学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2015-05-20