| Project/Area Number |
15K10874
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Nagoya City University |
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Principal Investigator |
AKI KATO 名古屋市立大学, 大学院医学研究科, 講師 (60405157)
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| Co-Investigator(Kenkyū-buntansha) |
安川 力 名古屋市立大学, 大学院医学研究科, 准教授 (00324632)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 加齢黄斑変性 / 網膜色素上皮 / ブルッフ膜 / スフェロイド / Ｇプロテイン / スフェロイド形成 / タイムラプス / Ｇタンパク質 |
|---|
| Outline of Final Research Achievements |
Human retinal pigment epithelium (RPE) cells were cultured without growth factor. For making spheroids, human RPE cells were trypsinized, collected, and resuspended with methylcellulose solution in round-bottom culture plate. Spheroids were collected every day and fixed in in 4% paraformaldehyde, thereafter, the expression of actin was evaluated by immunohistochemistry. Suspended human RPE cells with methylcellulose solution in round-bottom culture plate has been imaged in long-term time-lapse movies over 48 hours. The shape and size of spheroids were analyzed at each time point. The effect of Rho-associated kinase (Rho-kinase) inhibitor for spheroids was evaluated by time-lapse imaging and expression of associated protein.
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