| Project/Area Number |
15K10980
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井上 一由 岡山大学, 医学部, 客員研究員 (10624413)
森松 博史 岡山大学, 医歯薬学総合研究科, 教授 (30379797)
高橋 徹 岡山県立大学, 保健福祉学部, 教授 (40252952)
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| Research Collaborator |
YAMAOKA Masakazu
OMORI Emiko
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 集中治療学 / 急性腎不全 / 横紋筋融解症 / ヘムオキシゲナーゼー１ / Bach1 / オートファジー / ヘムオキシゲナーゼ / ヘムオキシゲナーゼ－１ / Nrf2 / ヘムオキシゲナーゼ-１ |
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| Outline of Final Research Achievements |
We demonstrate that nuclear Bach1 protein was rapidly and significantly decreased in the kidneys of rats with glycerol-associated RM-AKI, followed by an increase in Bach1 protein in the cytosol, which was preceded by the induction of Bach1 mRNA. We detected a significant increase in HO-1 expression and the robust inhibition of ALAS1 expression in the kidneys of glycerol-treated animals, suggesting a significant increase in the free
heme concentration in the kidney of glycerol-treated animals. Bach1 is a heme responsive transcription repressor of the HO-1 gene, and our findings suggest that changes in the subcellular distribution of Bach1 may be involved in the induction of HO-1 accompanying heme metabolism in the kidney of the rat RM-AKI model. To the best of our knowledge, this is the first study to show dynamic changes in renal Bach1 expression in vivo, which were associated with heme metabolism.
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