In silico screening of Interleukin-15 mimetics and application for a treatment of elderly septic patients

• Project/Area Number: 15K10994
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Tokai University
• Principal Investigator: INOUE Shigeaki 東海大学, 医学部, 准教授 (30582209)
• Co-Investigator(Kenkyū-buntansha): 平山 令明 東海大学, 先進生命科学研究所, 教授 (70238393)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: IL-15 / In silico screening / 老化 / 免疫疲弊 / 敗血症 / 炎症 / ミメティクス / 免疫

Outline of Final Research Achievements

Interleukin-15 (IL-15) is a cytokine that acts on NK cells and T cells and induces cell proliferation and activation. Evaluation of the effect on STAT5 phosphorylation of 15 compounds selected by in silico screening showed strong antagonistic (inhibitory) effect (inhibition rate 32%) with compound N43. In addition, weak inhibitory effect was observed for compounds N6, N15 and N35. In future, we aim to establish an evaluation system utilizing a sandwich ELISA method in the future.