| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We created mice with double deletion of Rac1 and Cdc42. We aimed to study the functional duplication of Rac1 and Cdc42 genes by comparing their phenotypes with those of mice. In order to specifically delete the Rac1 and Cdc42 genes in cartilage tissue, Rac1 flox mice Cdc42 flox mice and Col2 Cre transgenic mice were mated. The mice in which the Rac1 and Cdc42 genes were double deleted became lethal soon after birth, as in the case where the Cdc42 gene was single deleted. The long bones were shorter compared to the single deletion of the Rac1 and Cdc42 genes. In addition, the mice in which the Rac1 and Cdc42 genes were double deleted were found to have abnormal formation in skeletal organs. Stronger abnormalities were observed in cell composition and columnar formation in the growth plate than those of Rac1 and Cdc42 single knockout mice.
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