| Project/Area Number |
15K11110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Medical and Dental University (2016-2017)
Niigata University (2015) |
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Principal Investigator |
KANEKO Tomoatsu 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (70345297)
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| Research Collaborator |
Nör Jacques E. 米国ミシガン大学, 歯学部う蝕学・歯内治療学, 教授
Jin Qiming 米国ミシガン大学, 歯学部歯周病学, 講師
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯髄再生 / 歯髄組織再生 / 歯髄幹細胞 / 血管内皮細胞 |
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| Outline of Final Research Achievements |
In present analysis was performed by using in vivo protocol for pulp tissue engineering in pulpotomized rat teeth using implantation of stem cells. This model showed complete regeneration/healing with dentin bridge like formation within 2 weeks. First, dental pulp stem cells and endothelial cells from rat molars or incisors were corrected and cultured. Second, in vitro co-culture was performed and we found VEGF is one of key factors of cell growth of stem cell and/or endothelial cells. Finally, the implantation of stem cells and endothelial cells treated with VEGF into rat molars was performed. This model showed dental pulp tissue regeneration/healing within 1 week.
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