Risk diagnosis of cerebral infarction by metagenomic and anti-ApoH antibody analysis in apical periodontitis

• Project/Area Number: 15K11122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: Kato Satsuki 北海道医療大学, 歯学部, 講師 (50281283)
• Co-Investigator(Kenkyū-buntansha): 長澤 敏行 北海道医療大学, 歯学部, 教授 (90262203)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 根尖性歯周炎 / 動脈硬化症 / ApoH / TLRVYK / メタゲノム解析

Outline of Final Research Achievements

The aim of this study was to examine of ant-phospholipid antibody and TLRVYK antibody in apical periodontitis for systemic immune response by bacterial infection. Antibody titer of TLRVYK in systemic disease person were significant increase in comparison with healthy person. On the other hand, no differences of ant-phospholipid antibody between systemic disease person and healthy person. Next, we examined antibody titer of TLRVYK in apical periodontitis. Antibody titer of TLRVYK in apical periodontitis were higher than pulptitis. Furthermore, large number of bacteria detected in apical periodontitis by metagenomic analysis. Our results suggest that antibody titer of TLRVYK may be related with systemic immune response.

Research Products

• [Journal Article] Relationship between periodontitis and cardiovascular diseases via the immune responses against phospholipids (2016)
  • Author(s): 長澤敏行、白井 要、河野 舞、加藤幸紀、森 真理、古市保志
  • Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Volume: 58 Issue: 3 Pages: 98-106
  • DOI: 10.2329/perio.58.98
  • NAID: 130005268282
  • ISSN: 0385-0110, 1880-408X
  • Peer Reviewed / Open Access
• [Presentation] Ligature-induced Periodontitis Exacerbates Rheumatoid Arthritis in SKG Mice (2017)
  • Author(s): Matsumoto K, Nakatsuka Y, Kato S, Shimizu S, Furuichi Y
  • Organizer: 2017 IADR/AADR/CADR General Session & Exhibition
  • Place of Presentation: アメリカ合衆国サンフランシスコ
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Ligature induced periodontitis exacerbates reumatoid arthritis in SKG mice. (2017)
  • Author(s): 1.Kousei Matsumoto, Yuko Nakatsuka, Kaname Shirai, Shintaro Shimizu, Satsuki Kato, Yasusi Furuichi
  • Organizer: 5th General Session & Exhibition of the International Association for Dental Research
  • Int'l Joint Research
• [Presentation] 妊婦の口腔微生物叢の解析－ビフィズス菌の口腔・腸管・膣への細菌分布について－ (2016)
  • Author(s): 加藤幸紀、清水伸太郎、長澤敏行、中村梢、野口和行、古市保志
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-10-22
• [Presentation] 高脂血症患者における抗酸化物質と Porphyromonas gingivalis の 関係について (2016)
  • Author(s): 渡邉裕之，長澤敏行，清水伸太郎，寺田 裕，加藤幸紀，森 真理，川上智史，辻 昌宏，古市保志
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-10-22
• [Presentation] 妊婦の口腔微生物叢の解析－口腔・腸管・膣への細菌分布について－ (2016)
  • Author(s): 加藤幸紀、清水伸太郎、長澤敏行、中村梢、野口和行、波多江正紀、柿木博成、古市保志
  • Organizer: 第59回秋季日本歯周病学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2016-10-07
• [Presentation] Association of salivary P. gingivalis with serum antioxidants in hyperlipidemia patients (2016)
  • Author(s): Watanabe H, Nagasawa T, Shimizu S, Terada Y, Kato S, Konishi Y, Mori M, Maida T, Kawakami T, Ide H, Tsuji M, Furuichi Y
  • Organizer: The 102nd Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology and Japanese Academy of Clinical Periodontology
  • Place of Presentation: アメリカ合衆国サンディエゴ
  • Year and Date: 2016-09-10
  • Int'l Joint Research
• [Presentation] 妊婦と非妊娠女性における口腔微生物叢の解析 (2016)
  • Author(s): 加藤幸紀、清水伸太郎、長澤敏行、中村 梢、野口 和行、古市保志
  • Organizer: 第59回春季日本歯周病学会学術大会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2016-05-20
• [Presentation] 歯周組織の状態と高脂血症及び糖尿病との関連 (2016)
  • Author(s): 清水伸太郎、長澤敏行、渡邉裕之、寺田 裕、小西ゆみ子、森 真理、舞田健夫、川上智史、井出 肇、辻 昌宏、古市保志
  • Organizer: 第59回春季日本歯周病学会学術大会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2016-05-20
• [Presentation] 糖尿病を有する高脂血症患者における尿酸とP.gingivalisの関係 (2016)
  • Author(s): 渡邉裕之、長澤敏行、清水伸太郎、寺田 裕、加藤幸紀、小西ゆみ子、 森 真理、舞田健夫、川上智史、井出 肇、辻 昌宏、古市保志
  • Organizer: 第59回春季日本歯周病学会学術大会
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2016-05-20