The development of novel drug promoting dentin formation by odontoblast intercellular signal transduction factors (ATP/glutamate)

• Project/Area Number: 15K11129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: Kimura Maki 東京歯科大学, 歯学部, 講師 (90582346)
• Co-Investigator(Kenkyū-buntansha): 渋川 義幸 東京歯科大学, 歯学部, 教授 (30276969) ; 田崎 雅和 東京歯科大学, 歯学部, 教授 (40155065) ; 佐藤 正樹 東京歯科大学, 歯学部, 講師 (80598855)
• Research Collaborator: KOJIMA yuki ; HIGASHIKAWA asuka ; NISHIYAMA akihiro ; SHIOZAKI yuta ; SATOU ryouichi
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 象牙芽細胞 / アルカリ / 石灰化 / アルカリ刺激 / SOCE

Outline of Final Research Achievements

In the present research project, results obtained by mineralization assay are as follows: 1) Alkaline stimuli to the human odontoblasts increased their mineralization levels, 2) TRPA1 channels mediated mineralization mechanism under both extracellular physiologic and high pH conditions in the odontoblasts.
In the intracellular Ca2+ concentration measurements, we obtained following results: 1) Odontoblasts expressed store-operated Ca2+ entry (SOCE) via an activation of Ca2+ release-activated Ca2+ channels, 2) Activation of P2Y or muscarinic cholinergic receptors induced SOCE in odontoblasts, 3) Alkaline stimuli increased the amplitude of SOCE in odontoblasts.

Academic Significance and Societal Importance of the Research Achievements

本研究で、アルカリ刺激により象牙芽細胞に生じるCa2+シグナルとそのCa2+シグナルの結果放出された象牙芽細胞間連絡因子が近傍象牙芽細胞に誘発させるCa2+シグナルを明らかにした。この成果の学術的意義は、歯科臨床で多用される水酸化カルシウム製剤・MTAなどの高Ca 含有アルカリ性製剤の象牙質形成促進作用メカニズムの一端を解明したことである。本研究成果の社会的意義はより象牙質形成速度の速い新規高Ca含有アルカリ性製剤の開発につながることである。

Research Products

• [Journal Article] Intracellular Ca2+ mobilization pathway via bradykinin B1 receptor activation in rat trigeminal ganglion neurons. (2019)
  • Author(s): Terashima R, Kimura M, Higashikawa A, Kojima Y, Ichinohe T, Tazaki M, Shibukawa Y.
  • Journal Title: Journal of Physiological Sciences Volume: 69 Issue: 2 Pages: 199-209
  • DOI: 10.1007/s12576-018-0635-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High pH-sensitive store-operated Ca2+ entry mediated by Ca2+ release-activated Ca2+ channels in rat odontoblasts (2018)
  • Author(s): Kimura M., Nishi K., Higashikawa A., Ohyama S., Sakurai K., Tazaki M., Shibukawa1 Y.
  • Journal Title: Frontiers in Physiology Volume: - Pages: 443-443
  • DOI: 10.3389/fphys.2018.00443
  • Peer Reviewed / Open Access
• [Journal Article] Activation of mechanosensitve TRP/Piezo channels in odontoblasts generates action potentials in co-cultured IB4-negative medium-sized trigeminal ganglion neurons. (2018)
  • Author(s): Sato M, Ogura K, Kimura M, Nishi K, Ando M, Tazaki M, Shibukawa Y.
  • Journal Title: Journal of Endodontics Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Potassium Currents Activated by Depolarization in Odontoblasts (2017)
  • Author(s): Kojima Yuki、Kimura Maki、Higashikawa Asuka、Kono Kyosuke、Ando Masayuki、Tazaki Masakzu、Shibukawa Yoshiyuki
  • Journal Title: Frontiers in Physiology Volume: 8 Pages: 1078-1078
  • DOI: 10.3389/fphys.2017.01078
  • Peer Reviewed / Open Access
• [Journal Article] Temporal Expression Patterns of Clock Genes and Aquaporin 5/Anoctamin 1 in Rat Submandibular Gland Cells (2017)
  • Author(s): Satou Ryouichi、Sato Masaki、Kimura Maki、Ishizuka Yoichi、Tazaki Masakazu、Sugihara Naoki、Shibukawa Yoshiyuki
  • Journal Title: Frontiers in Physiology Volume: 8 Pages: 320-320
  • DOI: 10.3389/fphys.2017.00320
  • Peer Reviewed / Open Access
• [Journal Article] Ionotropic P2X ATP receptor channels mediate purinergic signaling in mouse odontoblasts. (2017)
  • Author(s): Shiozaki Y, Sato M, Kimura M, Sato T, Tazaki M, Shibukawa Y
  • Journal Title: Frontiers in Physiology Volume: 8
  • DOI: 10.3389/fphys.2017.00003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High pH-Sensitive TRPA1 Activation in Odontoblasts Regulates Mineralization (2016)
  • Author(s): Kimura M, Sase T, Higashikawa A, Sato M, Sato T, Tazaki M, Shibukawa Y
  • Journal Title: Journal of Dental Research Volume: 1 Issue: 9 Pages: 1-8
  • DOI: 10.1177/0022034516644702
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Intercellular signal communication among odontoblasts and trigeminal ganglion neurons via glutamate (2016)
  • Author(s): Nishiyama A, Sato M, Kimura M, Katakura A, Tazaki M, Shibukawa Y
  • Journal Title: Cell Calcium Volume: 60 Issue: 5 Pages: 341-355
  • DOI: 10.1016/j.ceca.2016.07.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction (2015)
  • Author(s): Shibukawa, Y., Sato, M., Kimura, M., Sobhan, U., Shimada, M., Nishiyama, A., Kawaguchi, A., Soya, M., Kuroda, H., Katakura, A., Ichinohe, T., and Tazaki, M.
  • Journal Title: Pflügers Archiv European Journal of Physiology Volume: 467 Issue: 4 Pages: 843-863
  • DOI: 10.1007/s00424-014-1551-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression and function of purinergic P2Y12 receptors in rat trigeminal ganglion neurons. (2015)
  • Author(s): Kawaguchi A, Sato M, Kimura M, Ichinohe T, Tazaki M, Shibukawa Y.
  • Journal Title: Neuroscience Research Volume: 98 Pages: 17-27
  • DOI: 10.1016/j.neures.2015.04.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Depolarization-induced Intracellular Free Calcium Concentration Increases Show No Desensitizing Effect in Rat Odontoblasts (2015)
  • Author(s): Kojima Y, Higashikawa A, Kimura M, Sato M, Mochizuki H, Ogura K, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M
  • Journal Title: The Bulletin of Tokyo Dental College Volume: 56 Issue: 2 Pages: 131-134
  • DOI: 10.2209/tdcpublication.56.131
  • NAID: 130005078126
  • ISSN: 0040-8891
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional expression of bradykinin B1 and B2 receptors in neonatal rat trigeminal ganglion neurons (2015)
  • Author(s): Kawaguchi A, Sato M, Kimura M, Yamazaki T, Yamamoto H, Tazaki M, Ichinohe T, Shibukawa Y
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 9 Pages: 229-229
  • DOI: 10.3389/fncel.2015.00229
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intercellular odontoblast communication via ATP mediated by pannexin-1 channel and phospholipase C-coupled receptor activation. (2015)
  • Author(s): Sato M, Furuya T, Kimura M, Kojima Y, Tazaki M, Sato T, Shibukawa Y.
  • Journal Title: Frontiers in Physiology Volume: 6 Pages: 326-326
  • DOI: 10.3389/fphys.2015.00326
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transient Receptor Potential Cation Channel Subfamily Vanilloid Member 3 is not Involved in Plasma Membrane Stretch-induced Intracellular Calcium Signaling in Merkel Cells (2015)
  • Author(s): Higashikawa A, Kojima Y, Sato M, Kimura M, Ogura K, Mochizuki H, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M.
  • Journal Title: The Bulletin of Tokyo Dental College Volume: 56 Issue: 4 Pages: 259-262
  • DOI: 10.2209/tdcpublication.56.259
  • NAID: 130005129286
  • ISSN: 0040-8891
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] P2X7 receptor-pannexin-1 channel interaction in rat trigeminal ganglion neuron (2019)
  • Author(s): Hiroyuki Inoue, Hidetaka Kuroda, Noboru Ishikawa, Sadao Ohyama, Asuka Higashikawa, Maki Himura, Hitoshi Yamamoto, Yoshiyuki Shibukawa, Tatsuya Ichinohe
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies congress (FAOPS), 第96回日本生理学会大会
  • Int'l Joint Research
• [Presentation] Intracellular cAMP induces Ca2+ influx in odontoblasts (2019)
  • Author(s): Maki Kimura, Asuka Higashikawa, Sadao Ohyama, Wataru Ofusa, Miyuki Shimada, Hidetaka Kuroda, Hiroyuki Mochizuki, Masayuki Ando, Kyosuke Kono, Yoshiyuki Shibukawa
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies congress (FAOPS), 第96回日本生理学会大会
  • Int'l Joint Research
• [Presentation] Voltage-dependent Ionic Channels in Human Cementoblast (2019)
  • Author(s): Satomi Kamata, Asuka Higashikawa, Maki Kimura, Sadao Oyama, Yoshiyuki Shibukawa, Shuichiro Yamashita
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies congress (FAOPS), 第96回日本生理学会大会
  • Int'l Joint Research
• [Presentation] Direct Mechanical stimulation evoked Gd3+-sensitive inward current in trigeminal ganglion neurons (2019)
  • Author(s): Asuka Higashikawa, Maki Kimura, Miyuki Shimada, Hidetaka Kuroda, Wataru Ofusa, Sadao Ohyama, Masayuki Ando, Kyousuke Kono, Hiroyuki Mochizuki, Yoshiyuki Shibukawa
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies congress (FAOPS), 第96回日本生理学会大会
  • Int'l Joint Research
• [Presentation] Expression of Mechanosensitive Ion Channel in Osteoblasts (2019)
  • Author(s): Sayoko Nagai, Asuka Higashikawa, Sadao Ooyama, Maki Kimura, Yoshiyuki Shibukawa, Akira Katakura
  • Organizer: 9th Federation of the Asian and Oceanian Physiological Societies congress (FAOPS), 第96回日本生理学会大会
  • Int'l Joint Research
• [Presentation] ヒト由来セメント芽細胞の電位依存性イオンチャネル発現 (2018)
  • Author(s): 鎌田聡仁，東川明日香，木村麻記，澁川義幸，山下秀一郎
  • Organizer: 第305回東京歯科大学学会
• [Presentation] Activation of Gs-protein-coupled receptors increases intracellular cAMP level in odontoblasts (2018)
  • Author(s): Maki Kimura, Asuka Higashikawa, Sadao Ohyama, Hiroyuki Mochizuki, Kyosuke Kouno, Masayuki Ando, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Organizer: 96th General Session & Exhibition of the International Association for Dental Research
  • Int'l Joint Research
• [Presentation] Light Conditions Affect Rhythmic Expression of Ano1 in Submandibular Glands (2018)
  • Author(s): Ryouichi Satou, Maki Kimura, Yoichi Ishizuka, Seitaro Suzuki, Yoshiyuki Shibukawa and Naoki Sugihara
  • Organizer: 96th General Session & Exhibition of the International Association for Dental Research
  • Int'l Joint Research
• [Presentation] 象牙芽細胞においてGsタンパク質共役型受容体活性化は細胞内cAMPレベルを増加する (2018)
  • Author(s): 木村麻記，東川明日香，大山定男，大房航，隝田みゆき，黒田英孝，望月浩幸，安藤正之，河野恭佑，澁川義幸
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] 象牙質痛は象牙芽細胞と歯髄ニューロンの神経性伝達で生じる (2018)
  • Author(s): 大山定男，人見涼露，東川 明日香，大房航，望月浩幸，安藤正之，河野恭佑，黒田英孝，木村 麻記，小野 堅太郎，渋川 義幸
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] 三叉神経節ニューロンにおけるP2X7 受容体-パネキシンチャネルカップリング (2018)
  • Author(s): 井上博之，黒田英孝，大山定男，大房航，永井佐代子，鎌田聡仁，東川明日香，木村麻記，澁川義幸，一戸達也
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] 新生仔ラット三叉神経節細胞における機械刺激受容 (2018)
  • Author(s): 東川 明日香，木村 麻記，隝田 みゆき，大房航， 大山定男，安藤正之，河野恭佑，望月浩幸，渋川義幸
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] 骨芽細胞の機械感受性イオンチャネル発現 (2018)
  • Author(s): 永井佐代子，東川明日香，大山定男，大房航，望月浩幸，安藤正之，河野恭祐，木村麻記，澁川義幸，片倉朗
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] ヒト由来セメント芽細胞の電位依存性イオンチャネル発現 (2018)
  • Author(s): 鎌田聡仁，東川明日香，木村麻記，井上博之，大山定男，大房 航，澁川義幸，山下秀一郎
  • Organizer: 第60回歯科基礎医学会学術大会
• [Presentation] Comparison of electrophysiological properties of P2X7 receptors between neurons and glial cells in rat trigeminal ganglion (2018)
  • Author(s): Inoue Hiroyuki, Kuroda Hidetaka, Ishikawa Noboru, Kimura Maki, Shibukawa Yoshiyuki, Tazaki Masakazu, Ichinohe Tatsuya
  • Organizer: 第95回日本生理学会大会
• [Presentation] Odontoblasts express plasma membrane Ca2+-ATPase 1 and 4 (2018)
  • Author(s): Kimura Maki, Higashikawa Asuka, Kojima Yuki, Terashima-Shigefuji Reiko, Inoue Hiroyuki, Nagai Sayoko, Kamata Satomi, Shimada Miyuki, Kuroda Hidetaka, Ogura Kazuhiro, Mochizuki Hiroyuki, Kouno Kyosuke, Ando Masayuki, Shibukawa Yoshiyuki, Tazaki Masakazu
  • Organizer: 第95回日本生理学会大会
• [Presentation] Alkali- and ADP-sensitive store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts (2017)
  • Author(s): Maki Kimura, Masaki Sato, Yuki Kojima, Asuka Higashikawa, Yuuta Shiozaki, Ryoichi Satou, Reiko Shigefuji, Miyuki Shimada, Hidetaka Kuroda, Kazuhiro Ogura, Hiroyuki Mochizuki, Kyosuke Kouno, Yoshiyuki Shibukawa, Masakazu Tazaki
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 第94回日本生理学会大会
  • Year and Date: 2017-03-28
• [Presentation] 象牙芽細胞における高pH感受性store-operated Ca2+ entry（SOCE） (2017)
  • Author(s): 木村麻記，小島佑貴，東川明日香，大山定男，隝田みゆき，安藤正之，河野恭祐，田崎雅和，澁川義幸
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] 三叉神経節ニューロンに対するグアヤコールの鎮痛作用 (2017)
  • Author(s): 隝田みゆき，木村麻記，佐藤正樹，東川明日香，小島佑貴，田崎雅和，澁川義幸
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] ラット三叉神経節細胞におけるP２X７受容体の発現検索 (2017)
  • Author(s): 井上博之，黒田英孝，小島佑貴，重藤玲子，東川明日香，石川昂，木村麻記，佐藤正樹，澁川義幸，田﨑雅和，一戸達也
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] ラット三叉神経節グリア細胞の遅延整流型K+電流 (2017)
  • Author(s): 小島佑貴，東川明日香，大山定男，河野恭佑，安藤正之，西孝一，黒田英孝，木村麻記，澁川義幸，田﨑雅和
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] メルケル細胞-神経細胞間コミュニケーション (2017)
  • Author(s): 東川明日香，小島佑貴，寺島‐重藤玲子，井上博之，河野恭佑，安藤正之，黒田英孝，木村麻記，澁川義幸，田﨑雅和
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] ラット三叉神経節ニューロンにおけるブラジキニンB１受容体を介したCa2+動態の検索 (2017)
  • Author(s): 寺島-重藤玲子，東川明日香，小島佑貴，井上博之，川口綾，黒田英孝，木村麻記，澁川義幸，田崎雅和，一戸達也
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] 内舌筋活動記録の試み－磁石を利用した電極を用いて (2016)
  • Author(s): 大房航，山田好秋，平木圭佑，佐藤正樹，木村麻記，澁川義幸，田﨑雅和
  • Organizer: 第10回三叉神経領域の感覚－統合機能研究会
  • Place of Presentation: 佐久市
  • Year and Date: 2016-11-26
• [Presentation] 象牙芽細胞に発現するTRPA1チャネルはアルカリ感受性を示し石灰化を促進する (2016)
  • Author(s): 木村麻記，佐藤正樹，篠宏美，小野寺晶子，東川明日香，小島佑貴，塩崎雄大，佐藤涼一，重藤玲子，隝田みゆき，小倉一宏，望月浩幸，河野恭佑，澁川義幸，田崎雅和
  • Organizer: 第10回三叉神経領域の感覚－統合機能研究会
  • Place of Presentation: 佐久市
  • Year and Date: 2016-11-26
• [Presentation] 象牙芽細胞の低pH感受性カルシウム動員 (2016)
  • Author(s): 佐藤正樹，倉澤馨，岡林堅，木村麻記，小島佑貴，東川明日香，河野恭佑，澁川義幸，田崎雅和
  • Organizer: 第10回三叉神経領域の感覚－統合機能研究会
  • Place of Presentation: 佐久市
  • Year and Date: 2016-11-26
• [Presentation] 象牙芽細胞におけるNK2受容体の発現の検索 (2016)
  • Author(s): 松浦信孝，木村麻記，佐藤正樹，小島佑貴，東川明日香，塩崎雄大，佐藤涼一，重藤玲子，田﨑雅和，澁川義幸
  • Organizer: 第23回歯科医学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-10-21
• [Presentation] 培養象牙芽細胞を用いた機械刺激受容に関する研究 (2016)
  • Author(s): 浅野一磨，佐藤正樹，木村麻記，小島佑貴，東川明日香，塩崎雄大，佐藤涼一，重藤玲子，澁川義幸，田﨑雅和
  • Organizer: 第23回歯科医学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-10-21
• [Presentation] 象牙芽細胞におけるアルカリ感受性store-operated Ca2+ entry（SOCE） (2016)
  • Author(s): 木村麻記，佐藤正樹，小島佑貴，東川明日香，隝田みゆき，小倉一宏，望月浩幸，河野恭佑，田崎雅和，澁川義幸
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] Guaiacol は三叉神経節ニューロンに作用し鎮痛効果を発揮する (2016)
  • Author(s): 隝田みゆき，木村麻記，佐藤正樹，東川明日香，小島佑貴，田崎雅和，澁川義幸
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] ラットTGニューロンにおける象牙芽細胞機械刺激誘発性活動電位記録 (2016)
  • Author(s): 佐藤正樹，木村麻記，小島佑貴，東川明日香，隝田みゆき，小倉一宏，望月浩幸，河野恭佑，澁川義幸，田崎雅和
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] High pH-sensitive TRPA1 channel activation acts as a key Ca2+ signaling that regulates dentin mineralization by odontoblasts (2016)
  • Author(s): Maki Kimura, Masaki Sato, Yuki Kojima, Asuka Higashikawa, Miyuki Shimada, Yuuta Shiozaki, Ryoichi Satou, Reiko Shigefuji, Kazuhiro Ogura, Hiroyuki Mochizuki, Kyosuke Kouno, Yoshiyuki Shibukawa, Masakazu Tazaki
  • Organizer: International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Store-operated Ca2+ entry in rat odontoblasts (2016)
  • Author(s): Maki Kimura, Asuka Higashikawa (presenter), Masaki Sato, Yuki Kojima, Miyuki Shimada, Yuuta Shiozaki, Ryoichi Satou, Reiko Shigefuji, Kazuhiro Ogura, Hiroyuki Mochizuki, Kyosuke Kouno, Yoshiyuki Shibukawa, Masakazu Tazaki
  • Organizer: International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Odontoblasts as sensory receptors: Transient receptor potential channels, pannexin-1 and ionotropic ATP receptors, mediate intracellular odontoblast-trigeminal ganglion neuron signal transduction (2016)
  • Author(s): oshiyuki Shibukawa, Masaki Sato, Maki Kimura, Ubaidus Sobhan, Yuki Kojima, Asuka Higashikawa, Yuuta Shiozaki, Ryoichi Satou, Reiko Shigefuji, Miyuki Shimada, Kazuhiro Ogura, Hiroyuki Mochizuki, Kyosuke Kouno, Masakazu Tazaki
  • Organizer: International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Functional expression of P2X receptor subtypes in odontoblasts (2016)
  • Author(s): Yuuta Shiozaki, Masaki Sato, Maki Kimura, Yoshiyuki Shibukawa, Masakazu Tazaki
  • Organizer: International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] ATP released from odontoblasts by mechanosensitive TRP and piezo channel activations generates P2X3-mediated action potentials in trigeminal ganglion neurons (2016)
  • Author(s): Masaki Sato, Maki Kimura, Yuki Kojima, Asuka Higashikawa, Yuuta Shiozaki, Ryoichi Satou, Reiko Shigefuji, Miyuki Shimada, Kazuhiro Ogura, Hiroyuki Mochizuki, Kyosuke Kouno, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Organizer: International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Biophysical properties of a Voltage-dependent K+ current in odontoblasts (2016)
  • Author(s): Yuki Kojima, Asuka Higashikawa, Maki Kimura, Masaki Sato, Yuuta Shiozaki, Ryoichi Satou, Reiko Shigefuji, Miyuki Shimada, Kazuhiro Ogura, Hiroyuki Mochizuki, Kyosuke Kouno, Yoshiyuki Shibukawa, Masakazu Tazaki
  • Organizer: International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] High pH-sensitive TRPA1 channel activation in odontoblasts regulates dentin mineralization and calcification (2016)
  • Author(s): Kimura M, Shibukawa Y, Sato M, Kojima Y, Higashikawa A, Kouno K, Shimada M, Mochizuki H, Ogura K, Tazaki M
  • Organizer: 12th International Confference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Porvoo, Finland
  • Year and Date: 2016-06-13
  • Int'l Joint Research
• [Presentation] voltage-dependent K+ current showing activation and inactivation kinetics in rat odontoblasts (2016)
  • Author(s): Kojima Y, Higashikawa A, Kimura M, Sato M, Mochizuki H, Ogura K, Shibukawa Y, Tazaki M
  • Organizer: 2th International Confference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Porvoo, Finland
  • Year and Date: 2016-06-13
  • Int'l Joint Research
• [Presentation] Expression of store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts (2016)
  • Author(s): Kimura M, Sato M, Kojima Y, Higashikawa A, Shiozaki Y, Satou R, Shigefuji R, Shimada M, Kobune K, Ogura K, Mochizuki H, Kouno K, Shibukawa Y, Tazaki M
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-03-22
• [Presentation] Evoked inward currents in trigeminal ganglion neurons caused by intracellular transmitters, ATP, released from mechanically stimulated odontoblasts (2016)
  • Author(s): Sato M, Kobune K, Kimura M, Kojima Y, Higashikawa A, Ogura K, Mochizuki H, Kouno K, Shimada M, Shibukawa Y, Tazaki M.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-03-22
• [Presentation] Merkel cells transduce mechanical stimulation into intercellular transmitter release (2016)
  • Author(s): Higashikawa A, Kojima Y, Kimura M, Sato M, Ogura K, Mochizuki H, Shibukawa Y, Tazaki M.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-03-22
• [Presentation] A voltage-dependent K+ current in rat odontoblasts (2016)
  • Author(s): Kojima Y, Higashikawa A, Kimura M, Sato M, Mochizuki H, Ogura K, Shibukawa Y, Tazaki M.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-03-22
• [Presentation] Functional expression of P2X receptors in an odontoblast cell line. (2016)
  • Author(s): Shiozaki Y, Sato M, Kimura M, Shibukawa Y, Sato T, Tazaki M.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-03-22
• [Presentation] Alkali stimulation-induced Ca2+ signaling in rat odontoblasts (2015)
  • Author(s): Shibukawa Y, Kimura M, Sato M, Kojima Y, Higashikawa A, Shimada M, Ogura K, Mochizuki H, Tazaki M
  • Organizer: 8th FAOPS congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] 三叉神経節ニューロンの象牙芽細胞機械刺激誘発性内向き電流の解析 (2015)
  • Author(s): 佐藤正樹，木村麻記，小島佑貴，東川明日香，西山明宏，塩崎雄大，佐藤涼一，重藤玲子，隝田みゆき，小倉一宏，望月浩幸，新谷明則，佐藤 亨，澁川義幸, 田﨑雅和
  • Organizer: 第300回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-10-17
• [Presentation] 象牙芽細胞におけるアルカリ刺激受容機構の解明 (2015)
  • Author(s): 佐瀬俊之，木村麻記，佐藤正樹，東川明日香，小島佑貴，佐藤 亨，澁川義幸, 田﨑雅和
  • Organizer: 第300回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-10-17
• [Presentation] メルケル細胞直接機械刺激による細胞内カルシウムシグナル (2015)
  • Author(s): 東川明日香，小島佑貴，木村麻記，佐藤正樹，小倉一宏，望月浩幸，澁川義幸，田﨑雅和
  • Organizer: 第300回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-10-17
• [Presentation] 象牙芽細胞におけるイオンチャネル型ATP 受容体サブタイプ（P２X）の機能的検索 (2015)
  • Author(s): 塩崎雄大，佐藤正樹，木村麻記，澁川義幸，佐藤亨，田﨑雅和
  • Organizer: 第300回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-10-17
• [Presentation] ラット三叉神経節ニューロンにおけるB１受容体を介したCa2+動態の評価 (2015)
  • Author(s): 重藤玲子，川口綾，佐藤正樹，木村麻記，澁川義幸，田﨑雅和，一戸達也
  • Organizer: 第300回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-10-17
• [Presentation] ラット象牙芽細胞における電位依存性K+チャネルの生物物理学的特性解析 (2015)
  • Author(s): 小島佑貴，東川明日香，木村麻記，佐藤正樹，小倉一宏，望月浩幸，澁川義幸，田﨑雅和
  • Organizer: 第300回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-10-17
• [Presentation] 象牙芽細胞におけるstore-operated Ca2+ entry（SOCE）（Ca2+ release-activated Ca2+（CARC）channels）の発現 (2015)
  • Author(s): 木村麻記，佐藤正樹，小島佑貴，東川明日香，しま田みゆき，小倉一宏，望月浩幸，田崎雅和，澁川義幸
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2015-09-11
• [Presentation] 象牙芽細胞機械刺激で誘発される三叉神経節ニューロン内向き電流：象牙芽細胞－ニューロン共培養によるodontoblast hydrodynamic receptor theoryの電気生理学的解析 (2015)
  • Author(s): 佐藤正樹，木村麻記，小島佑貴，東川明日香，しま田みゆき，小倉一宏，望月浩幸，澁川義幸，田崎雅和
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2015-09-11
• [Presentation] Activated mechanisms of H+-sensing ionotropic receptors and G-protein-coupled H+-sensing receptors in odontoblasts (2015)
  • Author(s): Sato M, Kurasawa K, Okabayashi K, Kimura M, Kojima Y, Higashikawa A, Shimada M, Ogura K, Mochizuki H, Sase T, Shinya A, Kobune K, Shibukawa Y and Tazaki M
  • Organizer: JOINT RESEARCH PROJECT (JSPS) UNDER THE JAPAN-KOREA BASIC SCIENTIFIC COOPERATION PROGRAM FOR FY 2015
  • Place of Presentation: 成田市
  • Year and Date: 2015-06-12
  • Invited
• [Presentation] Alkaline-sensitive intracellular Ca2+ signaling pathway in rat odontoblasts (2015)
  • Author(s): Kimura M, Sato M, Kojima Y, Higashikawa A, Nishiyama A, Satou R, Shiozaki Y, Shimada M, Ogura K,Mochizuki H, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M
  • Organizer: JOINT RESEARCH PROJECT (JSPS) UNDER THE JAPAN-KOREA BASIC SCIENTIFIC COOPERATION PROGRAM FOR FY 2015
  • Place of Presentation: 成田市
  • Year and Date: 2015-06-12
  • Invited
• [Presentation] ラット顎下腺由来唾液腺導管細胞株（SMIE）の時計遺伝子発現解析 (2015)
  • Author(s): 佐藤涼一，澁川義幸，石塚洋一，佐藤正樹，木村麻記，田﨑雅和，杉原直樹
  • Organizer: 第299回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-06-06
• [Presentation] TRPチャネルを介したATP放出は象牙芽細胞間情報連絡を担う (2015)
  • Author(s): 古屋 忠，佐藤正樹，木村麻記，小島佑貴，東川明日香，澁川義幸，田﨑雅和, 佐藤 亨
  • Organizer: 第299回東京歯科大学学会
  • Place of Presentation: 東京都
  • Year and Date: 2015-06-06
• [Presentation] ラット顎下腺導管細胞の時計遺伝子Bmal1発現リズム解析 (2015)
  • Author(s): 佐藤涼一、石塚洋一、杉原直樹、澁川義幸、佐藤正樹、木村麻記、
  • Organizer: 第65回日本口腔衛生学会・総会
  • Place of Presentation: つくば市
  • Year and Date: 2015-05-29