Regeneration of dental pulp/dentine composite by oral mucosal cells in novel hybrid scaffold

• Project/Area Number: 15K11140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KAKIGI Hideyuki 大阪歯科大学, 歯学部, 講師(非常勤) (40642830)
• Co-Investigator(Kenkyū-buntansha): 前田 博史 大阪歯科大学, 歯学部, 教授 (00274001) ; 好川 正孝 大阪歯科大学, 歯学部, 客員准教授 (70148451)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 口腔粘膜細胞 / Dexamethasone / β-Glycerophosphate / 二相性担体 / In vivo / In vitro / 歯の再生 / 間葉系幹細胞 / 多孔質担体 / オステオカルシン / 粘膜由来細胞 / シアノコバラミン / システイン / N-アセチル-L-システイン / 硬組織形成 / ハイブリッド担体 / アルギン酸スポンジ / ハイドロキシアパタイト / ポリビニルアルコールスポンジ / ポリ乳酸スポンジ / 歯髄・象牙質複合体 / 複合担体

Outline of Final Research Achievements

Addition of dexamethasone in culture medium of rat oral mucosal cells obtained induced small amount of calcified nodule deposition. The quantity was too small in comparison with bone marrow cells. This result suggested the possibility that oral mucosal cells might dedifferentiate to hard tissue forming cells. The factor which induced the proliferation of oral mucosa cells and those re-differentiation to hard tissue forming cells was examined. And cyanocobalamin revealed an effect.
Oral mucosa cells with seeding of a sponge made from alginic acid inserted in the cylindrical porous hydroxyapatite scaffold with a hollow center. In the subcutaneously implanted biphasic scaffold, a small amount of osteocalcin was detected. Submucosal connective tissue cells might differentiate to osteogenic cells.

Research Products

• [Journal Article] In vivo Estimation of Osteogenesis by Bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components (2016)
  • Author(s): Masataka Yoshikawa, Hideyuki Kakigi, Ayano Miyamoto, Sadaomi Sugimoto, Keisuke Nakai, Hideaki Ikenaga, Takeshi Inamoto, Hiroshi Maeda
  • Journal Title: Journal of Biomedical Science and Engineering Volume: 9 Issue: 11 Pages: 501-514
  • DOI: 10.4236/jbise.2016.911045
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bone formation in a scaffold composed of cylindrical hydroxyapatite and Tryptophan- or Lysine-coated sponge in vivo (2015)
  • Author(s): Masataka Yoshikawa, Hideyuki Kakigi, Hiroshi Maeda, Ikuo Nishikawa, Hideaki Ikenaga,Takeshi Inamoto, Norimasa Tsuji
  • Journal Title: Journal of Biomedical Science and Engineering Volume: 8 Issue: 06 Pages: 389-398
  • DOI: 10.4236/jbise.2015.86037
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] In vivo bone formation by bone marrow cells in bi-phasic scaffold consisting of porous hydroxyapatite with sponge (2017)
  • Author(s): Masataka Yoshikawa, Hideyuki Kakigi, Norimasa Tsuji, Takayoshi Yabuuchi, Hiroshi Maeda
  • Organizer: International Bone-tissue-engineering Congress
  • Int'l Joint Research
• [Presentation] 骨髄細胞による骨形成へのデキストランコーティングの効果 (2015)
  • Author(s): 宮本綾乃、杉本貞臣、中井啓介、前田博史、好川正孝、辻 則正、藪内祟督
  • Organizer: 日本歯科保存学会学術大会 平成27年度秋季大会（第143回）
  • Place of Presentation: 文京シビックホール、東京都
  • Year and Date: 2015-11-12
• [Presentation] In vivo bone formation by bone marrow cells in bi-phasic scaffold consisting of porous hydroxyapatite with sponge (2015)
  • Author(s): Masataka Yoshikawa, Hideyuki Kakigi, Norimasa Tsuji, Takayoshi Yabuuchi, Hiroshi Maeda
  • Organizer: International Bone-Tissue-Engineering Congress
  • Place of Presentation: Stuttgart, Germany
  • Year and Date: 2015-10-08
  • Int'l Joint Research