Establishment of order-made periodontal regenerative therapy based on micro RNA profile

• Project/Area Number: 15K11215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Iwata Tomoyuki 広島大学, 病院(歯), 助教 (30418793)
• Co-Investigator(Kenkyū-buntansha): 水野 智仁 広島大学, 病院(歯), 講師 (60325181) ; 兼田 英里 広島大学, 病院(歯), 歯科診療医 (30736426)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯周組織再生療法 / 間葉系幹細胞 / micro RNA / 転写因子 / 再生歯学 / サイトカイン / 歯周組織再生治療

Outline of Final Research Achievements

Some micro RNAs expressed characteristically in periodontal cells and mesenchymal stem cells (MSC) were identified and regulatory factors of micro RNA expression were also suggested.
From these results, in periodontal regenerative therapy with cell transplantation, selection of optimal regulatory factors based on each micro RNA profile of donor MSC can lead to enhancement of periodontal differentiation in transplantation site, finally, effective order made periodontal regenerative therapy can be established.

Research Products

• [Journal Article] Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration (2017)
  • Author(s): Kei Takeshita, Souta Motoike1, Mikihito Kajiya, Nao Komatsu, Manabu Takewaki, Kazuhisa Ouhara, Tomoyuki Iwata, Katsuhiro Takeda, Noriyoshi Mizuno, Tsuyoshi Fujita, and Hidemi Kurihara
  • Journal Title: Stem Cell Research & Therapy Volume: 8:101 Issue: 1 Pages: 101-101
  • DOI: 10.1186/s13287-017-0550-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] MSC/ECM Cellular Complexes Induce Periodontal Tissue Regeneration. (2017)
  • Author(s): Takewaki M, Kajiya M, Takeda K, Sasaki S, Motoike S, Komatsu N, Matsuda S, Ouhara K,
  • Journal Title: Journal of dental research Volume: 96(9) Issue: 9 Pages: 984-991
  • DOI: 10.1177/0022034517708770
  • Open Access
• [Presentation] 間葉系幹細胞から歯周組織構成細胞への分化制御遺伝子の探索 (2017)
  • Author(s): 岩田倫幸、永原隆吉、池田英里、八木亮一、間 悠介、小西昭弘、吉岡みなみ、石田 充、水野智仁、柴 秀樹、栗原英見
  • Organizer: 第59回秋季日本歯周病学会学術大会
  • Place of Presentation: 新潟市 朱鷺メッセ
  • Year and Date: 2017-10-07
• [Presentation] 間葉系幹細胞の細胞機能制御に関するmicro RNAの探索 (2017)
  • Author(s): 岩田倫幸、永原隆吉、兼田英里、吉岡みなみ、水野智仁、柴 秀樹、栗原英見
  • Organizer: 第59回春季日本歯周病学会学術大会
  • Place of Presentation: 鹿児島市 かごしま県民交流センター
  • Year and Date: 2017-05-20
• [Presentation] 歯周組織再生におけるmicro RNAによる間葉系幹細胞機能制御メカニズムの解明 (2017)
  • Author(s): 岩田 倫幸
  • Organizer: 第35回日本骨代謝学会学術集会