Analysis of the CTGF/CCN2 expression promotion system which classified cartilage regenerative therapy into the field

• Project/Area Number: 15K11247
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: Moritani Norifumi 岡山大学, 医歯薬学総合研究科, 助教 (60467751)
• Co-Investigator(Kenkyū-buntansha): 青山 絵理子 岡山大学, 医歯薬学総合研究科, 助教 (10432650) ; 滝川 正春 岡山大学, 医歯薬学総合研究科, 教授 (20112063) ; 西田 崇 岡山大学, 医歯薬学総合研究科, 准教授 (30322233) ; 松村 達志 岡山大学, 医歯薬学総合研究科, 講師 (70432648) ; 久保田 聡 岡山大学, 医歯薬学総合研究科, 教授 (90221936)
• Co-Investigator(Renkei-kenkyūsha): IIDA Seiji 岡山大学, 大学院医歯薬学総合研究科・顎口腔再建外科学分野, 教授 (40283791)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: CTGF/CCN2 / 軟骨再生 / RUNX2 / 鎖骨頭蓋異形成症

Outline of Final Research Achievements

CEBPB, CEBPD-adjusted altered expression of CTGF/CCN2, and their regulatory pathways are suggested to influence IL16 and COL12A1 activities. Interestingly, CEBPB and CEBPD were observed to develop in the nucleus and exhibit expression patterns similar to CTGF/CCN2. Results suggesting CEBPB and CEBPD activity in the CCN2 promoter region were also obtained. Furthermore, results suggesting that CEBPB and CEBPD accelerated proteoglycan synthesis were obtained when they were forcibly expressed. Finally, we also identified a RUNX2 gene frameshift mutation in a cleidocranial dysplasia patient and confirmed RUNX2 expression and CTGF/CCN2 localization using teeth tissue sections.

Research Products

• [Journal Article] A tumor suppressor gene product, platelet-derived growth factor receptor-like protein controls chondrocyte proliferation and differentiation. (2017)
  • Author(s): Kawata K, Kubota S, Eguchi T, Aoyama, E, Moritani N, Oka M, Kawaki H, and Takigawa M.
  • Journal Title: J Cell Biochem Volume: 118 Issue: 11 Pages: 4033-4044
  • DOI: 10.1002/jcb.26059
  • Peer Reviewed / Open Access
• [Journal Article] The influence of zoledronate and teriparatide on gamma delta T cells in mice (2017)
  • Author(s): Yamachika Eiki、Matsui Yuichi、Matsubara Masakazu、Matsumura Tatsushi、Nakata Naoki、Moritani Norifumi、Ikeda Atsushi、Tsujigiwa Hidetsugu、Ohara Naoya、Iida Seiji
  • Journal Title: Journal of Dental Science Volume: 12 Issue: 4 Pages: 333-339
  • DOI: 10.1016/j.jds.2017.03.007
  • Peer Reviewed / Open Access
• [Presentation] A familial case of cleidocranial dysplasia with a frameshift mutation in the RUNX2 gene. (2017)
  • Author(s): N. Moritani, Y. Yoshioka, E. Yamachika, Y. Matsui, M. Tabata, A. Ikeda, A. Uemura, K Nakatsuji, T. Matsumura, S. Iida.
  • Organizer: The 23nd International Conference on Oral & Maxillofacial Surgery (ICOMS)
  • Place of Presentation: Hong Kong
  • Year and Date: 2017-03-31
  • Int'l Joint Research
• [Presentation] A familial case of cleidocranial dysplasia with a frameshift mutation in the RUNX2 gene (2017)
  • Author(s): N. Moritani, Y. Yoshioka, E. Yamachika, Y. Matsui, M. Tabata, A. Ikeda, A. Uemura, K Nakatsuji, T. Matsumura, S. Iida
  • Organizer: he 23nd International Conference on Oral & Maxillofacial Surgery (ICOMS)
  • Int'l Joint Research