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Challenge to inferior alveolar nerve regeneration by human iPS cells for feeder-free and xeno-free system

Research Project

Project/Area Number 15K11267
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionKeio University

Principal Investigator

Kawana Hiromasa  慶應義塾大学, 医学部(信濃町), 准教授 (50224803)

Co-Investigator(Kenkyū-buntansha) 森川 暁  慶應義塾大学, 医学部(信濃町), 講師 (00424169)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords末梢神経再生 / 神経損傷 / 神経堤細胞 / iPS細胞 / 末梢神経損傷 / 細胞治療 / ゼノフリー培養法 / フィーダーフリー培養法 / 下歯槽神経再生 / フィーダーフリー / ゼノフリー
Outline of Final Research Achievements

The present study aims to induce neural crest cells from iPS cells and restore function of damaged nerve tissue. We established iPS cell culture method with feeder-free, xeno-free system for inducing neural crest cells. In order to investigate whether the induced neural crest cell population actually has the potential to restore peripheral nerve injury, we started the creation of the sciatic nerve cutting model and its functional evaluation. We found that transplanted induced neural crest cells possess the properties of mesenchymal stem cells and vascular endothelial cells that could also be induced into regenerating nerve tissues. These cells expressed peripheral nerve markers in transplantation tissues.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Applications of Mesenchymal Stem Cells and Neural Crest Cells in Craniofacial Skeletal Research.2016

    • Author(s)
      Morikawa S, Ouchi T, Shibata S, Fujimura T, Kawana H, Okano H, Nakagawa T.
    • Journal Title

      Stem Cells International

      Volume: 2849879 Issue: 1 Pages: 1-8

    • DOI

      10.1155/2016/2849879

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration.2015

    • Author(s)
      Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD, Morikawa S, Onizawa K, Kawana H, Akazawa C, Suzuki N, Nakagawa T, Okano H, Matsuzaki Y.
    • Journal Title

      journal of Dental Research

      Volume: 95 Issue: 2 Pages: 206-214

    • DOI

      10.1177/0022034515610748

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] LNGFR(+)THY-1(+) multipotent stem cells derived from human induced pluripotent stem cells2017

    • Author(s)
      Ouchi, T. Shibata, S. Kimura, H. Nagoshi, N. Fujimura, Y. Morikawa, S. Sato, K. Kawana, H. Fukuda, K. Nakamura, M. Nakagawa, T. Okano, H.
    • Organizer
      18th International Congress of Developmental Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neural Crest Cells Derived From Human iPS Cells: Differentiation, Multilineage Potential And Dramatic Fate2017

    • Author(s)
      Ouchi, T. Morikawa, S. Nakagawa, T. Kawana, H.
    • Organizer
      第62回公益社団法人日本口腔外科学会総会・学術大会.第2回 日中口腔顎顔面外科合同シンポジウム.
    • Related Report
      2017 Annual Research Report
  • [Presentation] Application of human induced pluripotent stem cell to craniofacial developmental biology and regenerative medicine2016

    • Author(s)
      Ouchi, T. Kawana, H. Morikawa, S. Okano, H Nakagawa, T.
    • Organizer
      66. Kongress & Praxisfuhrungsseminar der Deutschen Gesellschaft für Mund-, Kiefer- und Gesichtschirurgie
    • Place of Presentation
      Hamburg, Germany
    • Year and Date
      2016-06-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] バイオイメージング技術とコラーゲン及びスフェロイド形成を用いた in vivoトレーシングシステムの確立2016

    • Author(s)
      黄地健仁 森川暁 堀江伸行 河奈裕正 中川種昭
    • Organizer
      第70回NPO法人日本口腔科学会 学術集会
    • Place of Presentation
      福岡(福岡国際会議場)
    • Year and Date
      2016-04-15
    • Related Report
      2016 Research-status Report
  • [Book] the Quintessence 口腔外科 YEAR BOOK 一般臨床家,口腔外科医のための口腔外科ハンドマニュアル'15 口腔領域における再生医療の最前線① 口腔上皮の再生2015

    • Author(s)
      森川暁 黄地健仁 中川種昭 河奈裕正 岡野栄之
    • Total Pages
      5
    • Publisher
      クインテッセンス出版
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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