Challenge to inferior alveolar nerve regeneration by human iPS cells for feeder-free and xeno-free system

• Project/Area Number: 15K11267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: Kawana Hiromasa 慶應義塾大学, 医学部(信濃町), 准教授 (50224803)
• Co-Investigator(Kenkyū-buntansha): 森川 暁 慶應義塾大学, 医学部(信濃町), 講師 (00424169)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 末梢神経再生 / 神経損傷 / 神経堤細胞 / iPS細胞 / 末梢神経損傷 / 細胞治療 / ゼノフリー培養法 / フィーダーフリー培養法 / 下歯槽神経再生 / フィーダーフリー / ゼノフリー

Outline of Final Research Achievements

The present study aims to induce neural crest cells from iPS cells and restore function of damaged nerve tissue. We established iPS cell culture method with feeder-free, xeno-free system for inducing neural crest cells. In order to investigate whether the induced neural crest cell population actually has the potential to restore peripheral nerve injury, we started the creation of the sciatic nerve cutting model and its functional evaluation. We found that transplanted induced neural crest cells possess the properties of mesenchymal stem cells and vascular endothelial cells that could also be induced into regenerating nerve tissues. These cells expressed peripheral nerve markers in transplantation tissues.

Research Products

• [Journal Article] Applications of Mesenchymal Stem Cells and Neural Crest Cells in Craniofacial Skeletal Research. (2016)
  • Author(s): Morikawa S, Ouchi T, Shibata S, Fujimura T, Kawana H, Okano H, Nakagawa T.
  • Journal Title: Stem Cells International Volume: 2849879 Issue: 1 Pages: 1-8
  • DOI: 10.1155/2016/2849879
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration. (2015)
  • Author(s): Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD, Morikawa S, Onizawa K, Kawana H, Akazawa C, Suzuki N, Nakagawa T, Okano H, Matsuzaki Y.
  • Journal Title: journal of Dental Research Volume: 95 Issue: 2 Pages: 206-214
  • DOI: 10.1177/0022034515610748
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] LNGFR(+)THY-1(+) multipotent stem cells derived from human induced pluripotent stem cells (2017)
  • Author(s): Ouchi, T. Shibata, S. Kimura, H. Nagoshi, N. Fujimura, Y. Morikawa, S. Sato, K. Kawana, H. Fukuda, K. Nakamura, M. Nakagawa, T. Okano, H.
  • Organizer: 18th International Congress of Developmental Biology
  • Int'l Joint Research
• [Presentation] Neural Crest Cells Derived From Human iPS Cells: Differentiation, Multilineage Potential And Dramatic Fate (2017)
  • Author(s): Ouchi, T. Morikawa, S. Nakagawa, T. Kawana, H.
  • Organizer: 第62回公益社団法人日本口腔外科学会総会・学術大会．第2回 日中口腔顎顔面外科合同シンポジウム．
• [Presentation] Application of human induced pluripotent stem cell to craniofacial developmental biology and regenerative medicine (2016)
  • Author(s): Ouchi, T. Kawana, H. Morikawa, S. Okano, H Nakagawa, T.
  • Organizer: 66. Kongress & Praxisfuhrungsseminar der Deutschen Gesellschaft für Mund-, Kiefer- und Gesichtschirurgie
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 2016-06-01
  • Int'l Joint Research / Invited
• [Presentation] バイオイメージング技術とコラーゲン及びスフェロイド形成を用いた in vivoトレーシングシステムの確立 (2016)
  • Author(s): 黄地健仁 森川暁 堀江伸行 河奈裕正 中川種昭
  • Organizer: 第70回NPO法人日本口腔科学会 学術集会
  • Place of Presentation: 福岡（福岡国際会議場）
  • Year and Date: 2016-04-15
• [Book] the Quintessence 口腔外科 YEAR BOOK 一般臨床家，口腔外科医のための口腔外科ハンドマニュアル'15 口腔領域における再生医療の最前線① 口腔上皮の再生 (2015)
  • Author(s): 森川暁 黄地健仁 中川種昭 河奈裕正 岡野栄之
  • Total Pages: 5
  • Publisher: クインテッセンス出版