| Project/Area Number |
15K11358
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
沢 禎彦 岡山大学, 医歯薬学総合研究科, 教授 (70271666)
山崎 純 福岡歯科大学, 口腔歯学部, 教授 (50230397)
梶井 貴史 福岡歯科大学, 口腔歯学部, 准教授 (60322822)
中島 一記 福岡歯科大学, 口腔歯学部, 助教 (80610980)
秦 省三郎 福岡歯科大学, 口腔歯学部, 助教 (40736732)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ケラチノサイト / 線維芽細胞 / TGF-β１ / TRPV2 / ＴＲＰＶ２ / TGF-β1 / 歯科矯正学 |
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| Outline of Final Research Achievements |
Postoperative scar tissue on palates is considered to induce maxillary growth inhibition in cleft lip and palate patients. We characterized the role of TRP channels in the release of TGF-β1 from keratinocytes and the differentiation of fibroblasts to identify possible promising pharmacological approaches to prevent scar formation and contractures. The 3D culture model was made from rat skin seeded on a collagen gel in which dermal fibroblasts had been embedded. Among the TRP channel inhibitors tested, the TRPV2 inhibitors attenuated most potently keratinocyte-dependent and -independent collagen gel contraction due to TGF-β signaling as well as TGF-β1 release from keratinocytes and α-SMA production in myofibroblasts. TRPV2 was expressed in the epidermis and keratinocyte layers of the model. Compounds targeting TRPV2 channels would ameliorate wound contraction through the inhibition of TGF-β1 release and the differentiation of dermal fibroblasts in a culture model.
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