CO2 laser therapy accelerates the healing of ulcers in the oral mucosa
Project/Area Number |
15K11378
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthodontics/Pediatric dentistry
|
Research Institution | Fukuoka Dental College |
Principal Investigator |
OZAKI MASAO 福岡歯科大学, 口腔歯学部, 教授 (10152472)
|
Co-Investigator(Kenkyū-buntansha) |
岡 暁子 福岡歯科大学, 口腔歯学部, 准教授 (60452778)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | CO2 laser / Immunohistochemistry / Epithelial tissue / wound healing / tenascin C / CO2レーザー / 創傷治癒 / Heat Shock Protein / Tenascin C / マウス / 口腔粘膜 / 潰瘍 / 口蓋粘膜 / レーザー |
Outline of Final Research Achievements |
The present study used a mouse model of ulceration to investigate the molecular mechanisms by which CO2 laser therapy accelerated the wound healing process.Immunohistochemistry experiments showed that heat shock protein-70 (HSP70) was expressed mainly in the epithelium of normal palatal tissue, whereas there was little tenascin C (TnC) expression in the epithelium and mesenchyme under normal conditionsLaser irradiation induced HSP70 mRNA and protein expression in the lamina propria as well as TnC expression in the mesenchyme underlying the renewing epithelium.Culture of fibroblasts in heated medium increased the expressions of both TnC and TGF-β1, whereas laser irradiation induced only TnC expression.The present study indicates that CO2 laser irradiation exerts a photobiogenic effect to upregulate TnC expression without inducing TGF-β1 expression.We suggest that CO2 laser therapy has an advantage over thermal stimulation.
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Report
(4 results)
Research Products
(4 results)