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the unravelling of the role of cathepsin in gingival overgrowth

Research Project

Project/Area Number 15K11393
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Periodontology
Research InstitutionKyushu University

Principal Investigator

Yamashita Akiko  九州大学, 歯学研究院, 助教 (70511319)

Co-Investigator(Kenkyū-buntansha) 中西 博  九州大学, 歯学研究院, 教授 (20155774)
西村 英紀  九州大学, 歯学研究院, 教授 (80208222)
岩下 未咲  九州大学, 大学病院, 助教 (80611326)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords歯肉増殖症 / カテプシン / 歯肉増殖
Outline of Final Research Achievements

We previously suggested complete loss of Cathepsin-L function promoted gingival overgrowth.SPOCK-1,belonging to proteoglycan family, is a specific inhibitor of CtsL. The aim of this study is to investigate the role of SPOCK-1 in DIGO.
Human gingival fibroblasts were collected from patients who underwent flap operations.The cells were stimulated with Phenytoin (PHE), Cyclosporin A (CsA) or Nifedipine (NFE). The mRNA and protein levels of SPOCK-1 were examined by quantitative real time PCR and western blotting. As in vivo study, Spock-1 transgenic mice and wild-type littermates were generated, grown, and sacrificed. Gingival appearance was observed by microscopy. The results indicated that PHE, CsA and NFD promoted the expression of SPOCK-1 both at mRNA and protein level. The gingiva of Spock-1 transgenic mice exhibited different degree of fibrosis.SPOCK-1 upregulation was common among the three drug used, which may indicate the importance of SPOCK-1 in the pathophysiology of DIGO.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2016

All Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] 薬剤性歯肉増殖症におけるspock-1の役割2017

    • Author(s)
      Rehab Alshargabi, 高野愛子, 山下明子,豊田啓介, 岩下未咲,佐野朋美,竹下正章,鶴田満大,松永紘明,眞田大樹, 相田宜利,讃井彰一,西村英紀
    • Organizer
      平成29年度日本歯周病学会60周年記念京都大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Epicatechin down-regulates inflammatory genes in adipocytes co-cultured with endotoxin-activated macrophages.2017

    • Author(s)
      Akiko Yamashita, Tomomi Sano, Shintaro Nagayasu, Misaki Iwashita, Takanori Shinjo, Fusanori Nishimura
    • Organizer
      95th IADR General Session
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effect of cathepsin-B deficiency on gingival overgrowth in mice.2016

    • Author(s)
      Akiko Yamashita, Alshargabi Rehab Yahya Fara Sallam, Takanori Shinjyo, Misaki Iwashita, Hiroaki Matsunaga, Mitsudai Tsuruta, Yoko Hashimoto, Fusanori Nishimura
    • Organizer
      94th IADR General Session
    • Place of Presentation
      Seoul,Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effect of cathepsin-B deficiency on gingival overgrowth in mice.2016

    • Author(s)
      Alshargabi Rehab Yahya Fara Sallam, Akiko Yamashita, Takanori Shinjyo, Misaki Iwashita, Hiroaki Matsunaga, Mitsudai Tsuruta, Yoko Hashimoto, Fusanori Nishimura
    • Organizer
      第59回春季日本歯周病学会学術大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2016-05-21
    • Related Report
      2016 Research-status Report
  • [Presentation] The effect of cathepsin-B deficiency on gingival overgrowth in mice.2016

    • Author(s)
      Alshargabi Rehab Yahya Fara Sallam
    • Organizer
      2016 spring meeting of the Japanese Society of Periodontology
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-05-20
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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