Elucidation of periodontitis onset mechanism based on C1q and Wnt signaling

• Project/Area Number: 15K11394
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Fukuoka Dental College
• Principal Investigator: Yoshinaga Yausnori 福岡歯科大学, 口腔歯学部, 准教授 (60452869)
• Co-Investigator(Kenkyū-buntansha): 原 宜興 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (60159100)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯周ポケット形成 / 老化促進因子 / 動物モデル / C1q / Wnt / 老化 / 免疫感作

Outline of Final Research Achievements

To clarify the mechanism of periodontal onset, we investigated whether C1q and wnt signaling involved in the pocket formation using a rat periodotitis model.C1q was observed in the JE and in the connective tissue around the periodontal pocket in rat. Furthermore, C1qB was localized within the junctional epithelium around the periodontal pocket in long junctional epithelium rat model. These results suggest that C1q is involved in the pocket formation.

Academic Significance and Societal Importance of the Research Achievements

歯周病は日本人の7割以上が罹患している疾患であり、抜歯の原因の第一位である。その歯周炎の最大のリスクファクターである歯周ポケット形成への老化促進因子であるC1qの関与が示唆されたことは歯周ポケット形成メカニズムの解明において重要であり、ひいては歯周病の予防法や治療法の開発に強く寄与できると思われる。

Research Products

• [Journal Article] Topical application of glycyrrhetinic acid in the gingival sulcus inhibits attachment loss in lipopolysaccharide-induced experimental periodontitis in rats (2018)
  • Author(s): Takamori A.、Yoshinaga Y.、Ukai T.、Nakamura H.、Takamori Y.、Izumi S.、Shiraishi C.、Hara Y.
  • Journal Title: Journal of Periodontal Research Volume: 1 Issue: 3 Pages: 1-1
  • DOI: 10.1111/jre.12529
  • Peer Reviewed
• [Journal Article] Differentiation of Apical Bud Cells in a Newly Developed Apical Bud Transplantation Model Using GFP Transgenic Mice as Donor (2016)
  • Author(s): Maruo N, Sakagami R, Yoshinaga Y, Okamura K, Sawa Y.
  • Journal Title: PLoS One Volume: 11(3) Issue: 3 Pages: e0150766-e0150766
  • DOI: 10.1371/journal.pone.0150766
  • Peer Reviewed
• [Journal Article] The histopathological comparison on the destruction of the periodontal tissue between normal junctional epithelium and long junctional epithelium (2016)
  • Author(s): Noguchi S, Ukai T, Kuramoto A, Yoshinaga Y, Nakamura H, Takamori Y, Yamashita Y, Hara Y
  • Journal Title: Journal of Periodontal Research Volume: 印刷中 Issue: 1 Pages: 74-82
  • DOI: 10.1111/jre.12370
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] NLRP3インフラマソームは外傷性咬合による骨吸収に関与する (2018)
  • Author(s): 有田陽一, 吉永泰周, 金子高士, 河原ゆり, 中村恵子, 古賀千尋, 大城希美子, 山本南奈, 廣松亮, 有田晴一, 坂上竜資
  • Organizer: 第61回秋季日本歯周病学会
• [Presentation] グリブリドは歯周病原細菌によるラット実験的歯周炎を抑制する (2017)
  • Author(s): 吉永泰周，金子高士，有田陽一，河原ゆり，有田晴一，笠孝成，中村恵子，吉村篤利，古賀千尋，坂上竜資
  • Organizer: 第59回日本歯周病学会春季学術大会