Establishment of saliva marker for screening gingivitis.

• Project/Area Number: 15K11415
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Okayama University
• Principal Investigator: Takeuchi Mayu 岡山大学, 大学病院, 助教 (90745382)
• Co-Investigator(Kenkyū-buntansha): 森田 学 岡山大学, 医歯薬学総合研究科, 教授 (40157904) ; 江國 大輔 岡山大学, 医歯薬学総合研究科, 准教授 (70346443) ; 友藤 孝明 朝日大学, 歯学部, 教授 (80335629) ; 水谷 慎介 岡山大学, 医歯薬学総合研究科, 助教 (90643312)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 歯肉炎 / スクリーニング / 唾液LDH / 口腔細菌叢 / 次世代シーケンサー / MMP-9

Outline of Final Research Achievements

We investigated the usefulness of non-invasive screening for gingivitis using saliva. Among university students, we investigated the association between gingivitis and lactate dehydrogenase level in saliva in a cross-sectional study and the association between oral microflora in saliva and progression of gingivitis in a longitudinal study. The level of lactate dehydrogenase in saliva was significantly associated with gingivitis. However, there was no significant association between oral microflora and progression of gingivitis in university students.

Research Products

• [Journal Article] Association between gingivitis and related factors in Japanese university students using salivary lactate dehydrogenase level. (2018)
  • Author(s): Taniguchi-Tabata A, Ekuni D, Azuma T, Yoneda T, Yamane-Takeuchi M, Kataoka K, Mizuno H, Miyai H, Iwasaki Y, Morita M.
  • Journal Title: J Oral Sci. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Vallidity of a new kit measuring salivary lactate dehydrogenase level for screening gingivitis. (2017)
  • Author(s): Ekuni D, Yamane-Takeuchi M, KAtaoka K, Yokoi A, Taniguchi-Tabata A, Mizuno H, Miyai H, Uchida Y, Fukuhara D, Sugiura Y, Tomohuji T, Morita M.
  • Journal Title: Dis Markers. Volume: 2017:9547956. Pages: 1-6
  • DOI: 10.1155/2017/9547956
  • Peer Reviewed / Open Access
• [Journal Article] Validity of a New Kit Measuring Salivary Lactate Dehydrogenase Level for Screening Gingivitis (2017)
  • Author(s): Daisuke Ekuni, Mayu Yamane-Takeuchi, Kota Kataoka, Aya Yokoi, AyanoTaniguchi-Tabata, Hirofumi Mizuno, Hisataka Miyai, Yoko Uchida, Daiki Fukuhara, Yoshio Sugiura, Takaaki Tomofuji, Manabu Morita
  • Journal Title: Validity of a New Kit Measuring Salivary Lactate Dehydrogenase Level for Screening Gingivitis Volume: 2017 Pages: 6-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 歯肉炎のスクリーニングにおける唾液中LDH簡易測定キットの有効性 (2016)
  • Author(s): 吉田宗生、加藤壮真、竹内（山根）真由、江國大輔、横井彩、丸山貴之、山中玲子、 友藤孝明、森田 学
  • Organizer: 第65回日本口腔衛生学会・総会
  • Place of Presentation: 東京
  • Year and Date: 2016-05-27