| Project/Area Number |
15K11432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
IMAI TOSHIO 日本歯科大学, 生命歯学部, 教授 (90120617)
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| Co-Investigator(Kenkyū-buntansha) |
那須 優則 日本歯科大学, 生命歯学部, 教授 (50130688)
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| Co-Investigator(Renkei-kenkyūsha) |
YAEGAKI KEN 日本歯科大学, 生命歯学部, 教授 (40166468)
II HISATAKA 日本歯科大学, 生命歯学部, 講師 (00746604)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 口臭物質 / 歯槽骨吸収 / メカニカルストレス / 破骨細胞 / 骨芽細胞 / 細胞内シグナル伝達 / 硫化水素 / 骨髄幹細胞 |
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| Outline of Final Research Achievements |
It was aimed to elucidate the effect of mechanical stress on alveolar bone resorption by the oral malodorous compounds (hydrogen sulfide). Alveolar bone is routinely exposed to mechanical stress. A stretchable silicone vessel (SW) was used for the stretch stress. After coating SW with Poly-L-Lysine, Fibronectin, Collagen I, osteoclastic cells RAW 264 was seeded and induced the differentiation by RANKL.TRAP positive cells was observed only a little compared to the plastic dish.Cell proliferation and differentiation of osteoblastic cells MC3T3-E1 was observed in SW corted with Matrigel. It was suggested that ERK 1/2 plays an important role in its mechanism when hydrogen sulfide treated MC 3 T 3 - E 1 is on the stretch stress.
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