Transgenerational effects associated with radiation exposure in bulls after Fukushima accident

• Project/Area Number: 15K11952
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 復興農学
• Research Institution: Niigata University
• Principal Investigator: Hideaki Yamashiro 新潟大学, 自然科学系, 助教 (60612710)
• Co-Investigator(Kenkyū-buntansha): 杉村 智史 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (00728454)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 福島原発事故 / 被災雄牛 / 経世代 / エクソン解析 / メタボローム解析 / 被災雄牛由来子牛 / 精子 / 仔牛 / 継世代 / 雄性ゲノムクローニング

Outline of Final Research Achievements

We aimed to investigate the effect of exporting radiation for 6 months and 2 years in the ex-evacuation zone, a 20-km radius region surrounding the FNPP, on whole genome exome mutation of offspring associated with radiated bulls. As a result, there were no hereditary phenotypic alterations in the whole genome exome regions of calves associated with paternal exposure to ionizing radiation. Furthermore, we analysed transgenerational effects on calf on the blood plasma metabolome in order to detect alterations associated with paternal exposure to radiation. There were no significant transgenerational effects on the blood plasma metabolome in calf obtained by AI using sperm from LDR-radiated bull in the FNPP ex-evacuation zone. In calves produced by using sperm of Fukushima bulls, the radiation effects were extremely low. Thus, the results of our study would contribute to the reconstruction of the Fukushima livestock industry and facilitate devising food safety and security measures.

Research Products

• [Journal Article] Analysis of the Effect of Chronic and Low-Dose Radiation Exposure on Spermatogenic Cells of Male Large Japanese Field Mice (Apodemus speciosus ) after the Fukushima Daiichi Nuclear Power Plant Accident. (2016)
  • Author(s): Takino S, Yamashiro H, Sugano Y, Fujishima Y, Nakata A, Kasai K, Hayashi G, Urushihara Y, Suzuki M, Shinoda H, Miura T, Fukumoto M.
  • Journal Title: Radiat Res Volume: 187 Issue: 2 Pages: 161-168
  • DOI: 10.1667/rr14234.1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 被災雄牛における継世代影響評価 (2016)
  • Author(s): 山城秀昭, 高橋秀和, 藤 晋一, 漆原佑介, 桑原義和, 鈴木正敏, 阿部靖之, 杉村智史, 福田智一, 木野康志, 磯貝恵美子, 福本 学.
  • Journal Title: 無菌生物誌 Volume: 46 Pages: 22-23
• [Journal Article] Electron probe X-ray microanalysis of boar and inobuta testes after the Fukushima accident (2015)
  • Author(s): Yamashiro H, Abe Y, Hayashi G, Urushihara Y, Kuwahara Y, Suzuki M, Kobayashi J, Kino Y, Fukuda T, Tong B, Takino S, Sugano Y, Sugimura S, Yamada T, Isogai E, Fukumoto M.
  • Journal Title: Journal of Radiation Research Volume: 56 Issue: suppl 1 Pages: 42-47
  • DOI: 10.1093/jrr/rrv070
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Transgenerationaleffects on calf spermatogenesis and metabolomeassociated with paternal exposure to radiation after the Fukushima Nuclear Power Plant accident (2017)
  • Author(s): Yamashiro H, Fukumoto M
  • Organizer: 2017 International Symposium on Morphological Sciences
  • Invited
• [Presentation] 電子線マイクロアナライザによる被災アカネズミ精巣の元素分析 (2016)
  • Author(s): 大平拓也, 伊藤 洵, 菅原淳史, 山城秀昭,中田章史, 鈴木正敏, 有吉健太郎, 葛西宏介, 篠田 壽, 三浦富智, 福本 学.
  • Organizer: 日本放射線影響学会第59回大会
  • Place of Presentation: JMSアステールプラザ広島(広島県・広島市）
  • Year and Date: 2016-10-26
• [Presentation] 被災アカネズミにおける精巣のEPMA分析 (2016)
  • Author(s): 大平拓也, 伊藤 洵, 藤嶋洋平, 山城秀昭,中田章史, 鈴木正敏, 有吉健太郎, 葛西宏介, 篠田 壽, 三浦富智, 福本 学.
  • Organizer: 第3回 福島原発事故による周辺生物への影響に関する専門研究会
  • Place of Presentation: 京都大学原子炉実験所（大阪府・熊取町）
  • Year and Date: 2016-08-03
• [Presentation] 被災雄牛における継世代影響評価 (2016)
  • Author(s): 山城秀昭, 高橋秀和, 藤 晋一, 漆原佑介, 桑原義和, 鈴木正敏, 阿部靖之, 杉村智史, 福田智一, 木野康志, 磯貝恵美子, 福本 学
  • Organizer: 第49回無菌生物ノートバイオロジー学会
  • Place of Presentation: 仙台ガーデンパレス（宮城県・仙台市）
  • Year and Date: 2016-01-29
  • Invited
• [Presentation] 被災雄牛における継世代影響評価の試み (2015)
  • Author(s): 山城秀昭
  • Organizer: 福島原発事故による周辺生物への影響に関する専門研究会
  • Place of Presentation: 京都大学原子炉実験所（大阪府・熊取市）
  • Year and Date: 2015-08-10
• [Presentation] Effects of radiation exposure on transgenerational inheritance in bulls after the Fukushima Nuclear Power Plant accident (2015)
  • Author(s): Yamashiro H, Tong B, Takino S, Sugano Y, Tanaka S, Nishikawa T, Yoshida C, Abe Y, Hayashi G, Urushihara Y, Kuwahara Y, Suzuki M, Takahashi H, Fuji S, Isogai E, Fukumoto M
  • Organizer: 15th International Congress of Radiation Research (ICRR)
  • Place of Presentation: Kyoto International conference Centre (Kyoto Prefecture, Kyoto City)
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Book] 被災家畜における生殖器官・機能影響の評価 (2015)
  • Author(s): 山城秀昭、瀧野祥、菅野有晃、漆原佑介、鈴木正敏、桑原義和、阿部靖之、小林 仁、福田智一、木野康志、篠田 壽、磯貝恵美子、福本 学
  • Total Pages: 135
  • Publisher: 京都大学原子炉実験所レポート (KURRI Report)