Evaluation of radiation effect based on point mutation of BRAF gene

• Project/Area Number: 15K12202
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: The University of Tokyo
• Principal Investigator: Wada Youichiro 東京大学, アイソトープ総合センター, 教授 (10322033)
• Co-Investigator(Kenkyū-buntansha): KATSURA Mari 東京大学, アイソトープ総合センター, 特任助教 (30436571)
• Co-Investigator(Renkei-kenkyūsha): ISHIZAKI Azusa 独立行政法人日本原子力研究開発機構, 研究員 (10558894) ; AKIMITSU Nobuyoshi 東京大学, アイソトープ総合センター, 教授 (40294962) ; WILLOX Ralph 東京大学, 数理科学, 准教授 (20361610)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: がん遺伝子 / 生物影響

Outline of Final Research Achievements

BRAF V600E is likely a significant driving mutation in thyroid cancers, colorectal cancers and malignant melanomas. In this study, we have tried to develop a procedure to detect a single copy of point mutation, BRAF V600E among one hundred thousand copies to investigate the mechanisms of tumorigenesis. Using digital PCR system, we could determine the mutation in colon cancer cell line HCT116 treated with repeated high dose X-ray radiation, chronic low dose γ-ray radiation and Aristolochic acid. However, the frequencies of BRAF V600E were too small to determine whether they were caused by these treatments. Together, to investigate the formation of specific point mutation, we should further develop the procedure to analyze larger copy numbers than one hundred thousand and seek suitable biological conditions.

Research Products

• [Journal Article] Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells. (2016)
  • Author(s): Katsura M, Cyou-Nakamine H, Zen Q, Zen Y, Nansai H, Amagasa S, Kanki Y, Inoue T, Kaneki K, Taguchi A, Kobayashi M, Kaji T, Kodama T, Miyagawa K, Wada Y, Akimitsu N, Sone H.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 20027-20027
  • DOI: 10.1038/srep20027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Effects of Low- dose Internal Radiation From Cs-137 on Normal Human Cell (2015)
  • Author(s): Nobuyoshi Akimitsu, Mari Katsura, Azusa Ishibashi, Hiromasa Cyou Nakamine, Kazuyuki Tao, Toshiyuki Kaji, Hideko Sone, Tatsuhiko Kodama, Kiyoshi Miyagawa, Youichiro Wada
  • Organizer: ICRR
  • Place of Presentation: 京都国際会議場（京都府京都市）
  • Year and Date: 2015-05-25
• [Presentation] Effects of Low- and Middle- Dose Rate Radiation on Human Neural Precursor Cells (2015)
  • Author(s): Mari Katsura, Hiromasa Nakamine, Hideko Sone, Shota Amagasa, Toshiyuki Kaji, Kiyoshi Miyagawa, Youichirou Wada, Nobuyoshi Akimitsu
  • Organizer: ICRR
  • Place of Presentation: 京都国際会議場（京都府京都市）
  • Year and Date: 2015-05-25