Effects on the suppression of colorectal cancer cells by microRNA into nanovesicles in apple juice

• Project/Area Number: 15K12332
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Hirosaki University
• Principal Investigator: Chiba Mitsuru 弘前大学, 保健学研究科, 講師 (20583735)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: リンゴ / microRNA / ナノベシクル / 大腸癌

Outline of Final Research Achievements

Nanovesicles are extracellular vesicles that function as intercellular signal transduction factors. Recently, its existence has been clarified in fruit juice. I have clarified that a number of microRNAs exist in nanovesicles and induce functional changes on the cell. In this study, it was revealed that micro RNA and other small RNAs exist in nanovesicle contained in apple juice. In addition, it was revealed that nanovesicles containing RNA are taken up into colorectal cancer cell lines.