| Project/Area Number |
15K12345
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Eating habits
|
|---|
| Research Institution | The University of Shiga Prefecture |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 卵母細胞の質 / ビタミン / 欠乏 / 卵母細胞の減数分裂 / マウス / ビタミンB1 / 卵子の成熟 / 卵子の質 / 葉酸 |
|---|
| Outline of Final Research Achievements |
(1) The frequency of abnormal oocytes was no different between the FA-deficient and control groups (20.0% vs 14.6%). It was reported that FA transporter was strongly expressed in oocytes. It is thus possible that the mice fed FA-free diets may not affect the concentration of oocyte FA. In conclusion, our study suggested that FA deficiency did not adversely affect oocyte meiosis.
(2) Oocyte meiosis was not affected by vitamin B1 deficiency when the deficiency was not accompanied by body weight loss On the other hand, frequency of abnormal oocyte was increased by vitamin B1 deficiency when deficiency was accompanied by body weight loss (referred as severe vitamin B1 deficiency; frequency of abnormal oocyte, 13.8% vs 43.7%, P = 0.0071). The frequency of abnormal oocytes was decreased by refeeding of a vitamin B1-containing diet (13.9% vs 22.9%, P = 0.503). These results suggest that severe vitamin B1 deficiency inhibited meiotic maturation of oocytes, but did not damage immature oocytes.
|
