Investigation of alcohol toxicity and its prevention by functional food

Research Project

Project/Area Number	15K12364
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Eating habits
Research Institution	Tokai University
Principal Investigator	Nagai Ryoji 東海大学, 農学部, 教授 (20315295)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	アセトアルデヒド / 翻訳後修飾 / 飲酒マーカー / アルコール / 毒性 / 翻訳語修飾
Outline of Final Research Achievements	Acetaldehyde (AA), which is a metabolic intermediate of alcohol, shows toxicity by modification and denaturation of protein. In this study, AA modified amino acid was detected by LC-MS/MS in mouse serum and human serum after administration of alcohol. As this method is precise, this would be effective method to look into the association of AA and alcohol related diseases such as Alzheimer's disease and ischemic heart disease. Furthermore, we obtained monoclonal antibody against AA-modified proteins and this antibody could be useful tool to assess toxicity of AA in the near future.