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Development of new purification method of extracellular vesicles for cancer diagnostics

Research Project

Project/Area Number 15K12566
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Medical systems
Research InstitutionNational Institutes of Biomedical Innovation, Health and Nutrition

Principal Investigator

Kamada Haruhiko  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 創薬デザイン研究センター, プロジェクトリーダー (00324509)

Co-Investigator(Kenkyū-buntansha) 宇山 浩  大阪大学, 工学研究科, 教授 (70203594)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords分泌膜小胞 / バイオマーカー / エクソソーム / アフィニティ精製カラム
Outline of Final Research Achievements

New purification method of extracellular vesicle that derived from cancer cells was developed for the development of new diagnostic technique without using the human blood. We have identified the new biomarker proteins expressed on the extracellular vesicles and have created new monolith column for the purification of extracellular vesicles. These result indicated that this method is useful for collect the extracellular vesicles and will expect to utilize for development for new diagnostic method for cancer patients.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] A trimeric structural fusion of an antagonistic tumor necrosis factor-α mutant enhances molecular stability and enables facile modification2017

    • Author(s)
      Masaki Inoue, Daisuke Ando, Haruhiko Kamada, Shintaro Taki, Mayumi Niiyama, Yohei Mukai, Takashi Tadokoro, Katsumi Maenaka, Taisuke Nakayama, Yuji Kado, Tsuyoshi Inoue, Yasuo Tsutsumi and Shin-ichi Tsunoda
    • Journal Title

      J. Biol. Chem.,

      Volume: in press Issue: 16 Pages: 235-237

    • DOI

      10.1074/jbc.m117.779686

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of New Biologics through Creation of a Functional Cytokine Mutant2015

    • Author(s)
      鎌田 春彦
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 135 Issue: 7 Pages: 843-849

    • DOI

      10.1248/yakushi.15-00007-1

    • NAID

      130005085762

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
    • Open Access / Acknowledgement Compliant
  • [Presentation] 心不全に対する新規診断法の開発に向けたバイオマーカーの探索.2017

    • Author(s)
      大須賀 絵理, 東阪 和馬, 笠原 淳平, 豊田 麻人, 鎌田春彦, 前田 真貴子, 長野 一也, 藤尾 慈, 坂田 泰史, 堤 康央
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      宮城(仙台)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of Adaptor ProtreinsIncludedin the TNFR2 Signaling Complex2015

    • Author(s)
      Haruhiko Kamada
    • Organizer
      14th Human Proteome Organization World Congress
    • Place of Presentation
      Vancouver Convention Centre, Vancouver
    • Year and Date
      2015-09-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒトTNFR2指向性アゴニストを用いた新規TNFR2シグナル関連分子の同定2015

    • Author(s)
      鎌田 春彦,井上 雅己 ほか
    • Organizer
      日本プロテオーム学会 2015年会
    • Place of Presentation
      くまもと森都心プラザ,熊本
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Phage display as a powerful tool for drug discovery2015

    • Author(s)
      Haruhiko Kamada
    • Organizer
      ETH seminar
    • Place of Presentation
      Swiss Federal Institutes of Technology in Zurich, Zurich
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.nibiohn.go.jp/bio-r/index.htm

    • Related Report
      2016 Annual Research Report
  • [Remarks] バイオ創薬プロジェクト 公式ホームページ

    • URL

      http://www.nibiohn.go.jp/bio-r/index.htm

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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