Transformation and recognition of shape of lipid membrane by amphiphilic DNA nanostructures

• Project/Area Number: 15K12746
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Osaka University
• Principal Investigator: Dohno Chikara 大阪大学, 産業科学研究所, 准教授 (60420395)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: DNA / DNAナノ構造 / 両親媒性DNA / 膜変形 / 脂質二重膜 / リポソーム

Outline of Final Research Achievements

Lipid membranes play an important role in biological system. The lipid membranes define the boundaries of cell and various organelles, and their shape and physical properties are dynamically controlled in the living cells. We here propose a methodology to control the shape and mechanical properties of the lipid membranes by using nanoscale DNA objects made of membrane binding DNAs. We created a series of DNA nanostructures with various 2- and 3-dimensional shapes that efficiently bind to lipid membrane by introducing amphiphilic DNA strands containing hydrophobic functional groups. We demonstrated the binding of the DNA nanostructures could change a shape of liposome and mechanical properties of the lipid membranes.

Research Products

• [Int'l Joint Research] Oxford University(United Kingdom)
• [Journal Article] Amphiphilic DNA tiles for controlled insertion and 2D assembly on fluid lipid membranes: the effect on mechanical properties (2017)
  • Author(s): Dohno, C.; Makishi, S.; Nakatani, K.; Contera, S
  • Journal Title: Nanoscale Volume: 9 Issue: 9 Pages: 3051-3058
  • DOI: 10.1039/c6nr07084a
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A hybridisation-dependent membrane-insertable amphiphilic DNA (2015)
  • Author(s): Dohno, C.; Matsuzaki, K.; Yamaguchi, H.; Shibata, T.; Nakatani, K.
  • Journal Title: Org. Biomol. Chem. Volume: 13 Issue: 40 Pages: 10117-10121
  • DOI: 10.1039/c5ob01761k
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The evaluation of repeat DNA binding ligands having a hydrophobic functional group (2017)
  • Author(s): 山内和明・堂野主税・中谷和彦
  • Organizer: 日本化学会 第97春季年会
  • Place of Presentation: 慶應義塾大学（横浜市）
  • Year and Date: 2017-03-16
• [Presentation] Binding of amphiphilic DNA to lipid membrane regulated by formation of higher order structures (2016)
  • Author(s): Chikara Dohno, Hayato Yamaguchi, Shingo Makishi, Koichi Matsuzaki, Kazuhiko Nakatani
  • Organizer: The 43rd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 熊本大学（熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] 疎水性官能基を有する新規DNA結合リガンドの合成 (2016)
  • Author(s): 山内和明・堂野主税・中谷和彦
  • Organizer: 日本化学会第９６春季年会
  • Place of Presentation: 同志社大学京田辺キャンパス
  • Year and Date: 2016-03-24
• [Presentation] Modulation of binding properties of DNA assemblies to lipid bilayer membrane (2015)
  • Author(s): C. Dohno, S. Makishi, H. Yamaguchi, K. Nakatani
  • Organizer: Pacifichem2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Binding of amphiphilic DNAs with different secondary structures to lipid bi- layer membrane (2015)
  • Author(s): H. Yamaguchi, K. Matsuzaki, T. Shibata, C. Dohno, K. Nakatani
  • Organizer: Pacifichem2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research