Chemical probes for long time imaging of osteoclasts function by optimization of emission wavelength and photostability

• Project/Area Number: 15K12754
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Osaka University
• Principal Investigator: KIKUCHI Kazuya 大阪大学, 工学研究科, 教授 (70292951)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: in vivoイメージング / 蛍光プローブ / 破骨細胞 / 光安定性

Outline of Final Research Achievements

Chemical probes were developed for in vivo imaging of activated osteoclasts by using two-photon excitation microscopy. As fluorescent probes, rhodamine derivatives with pH-activatable property and red-shifted emission were designed and synthesized. The two-photon excitation microscopy images showed that fluorescence signals were observed at the specific regions in bone tissue in living mice.

Research Products

• [Journal Article] Photostable and Photoswitching Fluorescent Dyes for Super-resolution Imaging (2017)
  • Author(s): M. Minoshima, K. Kikuchi
  • Journal Title: Journal of Biological Inorganic Chemistry Volume: - Issue: 5 Pages: 1-14
  • DOI: 10.1007/s00775-016-1435-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Enzyme-Triggered Compound Release using Functionalized Antimicrobial Peptide Derivatives (2017)
  • Author(s): Shin Mizukami, Masayoshi Kashibe, Kengo Matsumoto, Yuichiro Hori, Kazuya Kikuchi
  • Journal Title: Chem. Sci. Volume: 8 Issue: 4 Pages: 3047-3053
  • DOI: 10.1039/c6sc04435b
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fluorogenic Probes Reveal a Role of GLUT4 N-glycosylation in Intracellular Trafficking (2016)
  • Author(s): S. Hirayama, Y. Hori, Z. Benedek, T. Suzuki, K. Kikuchi
  • Journal Title: Nature Chemical Biology Volume: 12 Issue: 10 Pages: 853-859
  • DOI: 10.1038/nchembio.2156
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Real-Time Intravital Imaging of pH Variation Associated with Osteoclast Activity (2016)
  • Author(s): Hiroki Maeda, Toshiyuki Kowada, Junichi Kikuta, Masayuki Furuya, Mai Shirazaki, Shin Mizukami, Masaru Ishii, Kazuya Kikuchi
  • Journal Title: Nat. Chem. Biol. Volume: 12 Issue: 8 Pages: 579-585
  • DOI: 10.1038/nchembio.2096
  • Peer Reviewed
• [Journal Article] Fabrication of ‘Clickable’ Polyfluorene Nanowires with High Aspect Ratio as Biological Sensing Platforms (2016)
  • Author(s): T. Wasin, K. Enomoto, T. Sakurai, V. Padalkar, H. Cheng, M. Tang, A. Horio, D., Sakamaki, M. Omichi, A. Saeki, K. Kikuchi, Y. Hori, A. Chiba, Y. Saito, T. Kamiya, M. Sugimoto, S. Seki
  • Journal Title: ACS Sensors Volume: 6 Issue: 6 Pages: 766-774
  • DOI: 10.1021/acssensors.6b00070
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Real-time intravital imaging of pH variation associated with osteoclast activity (2016)
  • Author(s): H. Maeda, T. Kowada, J. Kikuta, M. Furuya, M. Shirazaki, S. Mizukami, M. Ishii & K. Kikuchi,
  • Journal Title: Nature Chemical Biology Volume: 12
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Design of a Protein tag and Fluorogenic Probe with Modular Structure for Live-Cell Imaging of Intracellular Proteins (2016)
  • Author(s): Y. Kamikawa, Y. Hori, K. Yamashita, L. Jin, S. Hirayama, D.M. Standley & K. Kikuchi
  • Journal Title: Chemical Science Volume: 7 Issue: 1 Pages: 308-314
  • DOI: 10.1039/c5sc02351c
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Redesign of Fluorogenic Labeling System to Improve Surface Charges, Brightness, and Binding Kinetics for Imaging Functional Localization of Bromodomains (2015)
  • Author(s): Y. Hori, S. Hirayama, M. Sato & K. Kikuchi
  • Journal Title: Angewante Chemie International Edition Volume: 54 Issue: 48 Pages: 14368-14371
  • DOI: 10.1002/anie.201506935
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] BODIPY-based probes for the fluorescence imaging of biomolecules in living cells (2015)
  • Author(s): Toshiyuki Kowada, Hiroki Maeda, Kazuya Kikuchi
  • Journal Title: Chemical Society Reviews Volume: - Issue: 14 Pages: 4953-4972
  • DOI: 10.1039/c5cs00030k
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Real-time Intravital Imaging of pH Variation Associated with Osteoclast Activity Using BODIPY Based Two Photon Excitation Probes (2016)
  • Author(s): K. Kikuchi
  • Organizer: The 8th Asian Biological Inorganic Chemistry Conference (AsBIC VIII)
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2016-12-05
  • Int'l Joint Research / Invited
• [Presentation] Real-time Intravital Imaging of pH Variation Associated with Osteoclast Activity (2016)
  • Author(s): K. Kikuchi
  • Organizer: MSMLG 2016, 5th International Conference on Molecular Sensors and Molecular Logic Gates
  • Place of Presentation: Bath, UK
  • Year and Date: 2016-07-24
  • Int'l Joint Research / Invited
• [Presentation] Real-time Intravital Imaging of pH Variation Associated with Osteoclast Activity Using BODIPY Based Two Photon Excitation Probes (2016)
  • Author(s): K. Kikuchi
  • Organizer: ICPP-9, 9th International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Nanjing, China
  • Year and Date: 2016-07-03
  • Int'l Joint Research / Invited
• [Presentation] Development of Multifunctional 19F MRI Contrast Agents with Fluorine-encapsulated Silica Nanoparticle (2015)
  • Author(s): Kazuya Kikuchi
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Highly Sensitive 19F MRI Probes with Tunable Chemical Switches Using Metal Complexes (2015)
  • Author(s): Kazuya Kikuchi
  • Organizer: ICBIC-17, International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-07-20
  • Int'l Joint Research / Invited
• [Presentation] Development of Multifunctional 19F MRI Contrast Agents with Fluorine-encapsulated Silica Nanoparticle (2015)
  • Author(s): Kazuya Kikuchi
  • Organizer: 13th International Symposium on Applied Bioinorganic Chemistry (ISABC13)
  • Place of Presentation: Galway, Ireland
  • Year and Date: 2015-06-12
  • Int'l Joint Research / Invited