A study on the neural basis of sustained reversal movement

• Project/Area Number: 15K12769
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: Nagoya University
• Principal Investigator: Takagi Shin 名古屋大学, 理学研究科, 准教授 (90171420)
• Research Collaborator: Sugioka Takumi ; Kato Koharu
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 運動制御 / C. elegans / 光遺伝学 / 生体アミン / 後退運動 / 線虫 / 移動運動 / 神経回路

Outline of Final Research Achievements

Certain artificial stimuli can induce sustained backward locomotion (SBL) in animals. Taking advantage of the fact that optogenetic activation of a subset of neurons expressing the glr-1 gene induces sustained locomotion, we tried to elucidate the underlying cellular mechanisms. Activation of RIM neurons in addition to the command interneurons AVA and AVD is necessary for SBL. For longer SBL, activation of SMD and/or RMD appears to be critical. Although exogenous tyramine at a high concentration was reported to induce SBL, we found that tdc-1 mutants defective for the tyramine-synthesis performed longer SBL when glr-1 positive neurons were activated.

Research Products

• [Journal Article] Optical silencing of body wall muscles induces pumping inhibition in Caenorhabditis elegans (2017)
  • Author(s): Takahashi Megumi、Takagi Shin
  • Journal Title: PLOS Genetics Volume: 13 Issue: 12 Pages: 1007134-1007134
  • DOI: 10.1371/journal.pgen.1007134
  • Peer Reviewed / Open Access
• [Journal Article] Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP. (2017)
  • Author(s): Sherwood MW, Arizono M, Hisatsune C, Bannai H, Ebisui E, Sherwood JL, Panatier A, Oliet SH, Mikoshiba K.
  • Journal Title: Glia Volume: 65 Issue: 1 Pages: 502-513
  • DOI: 10.1016/j.bbrc.2016.09.034
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterizing Semaphorin Signaling in vivo using C. elegans (2017)
  • Author(s): Nukazuka A, Takagi S
  • Journal Title: Methods in Molecular Biology Volume: 1493 Pages: 485-498
  • DOI: 10.1007/978-1-4939-6448-2_34
  • ISBN: 9781493964468, 9781493964482
  • Peer Reviewed / Open Access
• [Journal Article] X-ray crystallographic structure of thermophilic rhodopsin: implications for high thermal stability and optogenetic function (2016)
  • Author(s): Tsukamoto T, Mizutani K, Hasegawa T, Takahashi M, Honda N, Hashimoto N, Shimono K, Yamashita K, Yamamoto M, Miyauchi S, Takagi S, Hayashi S, Murata T, Sudo Y.
  • Journal Title: J. Biol. Chem. Volume: in press Issue: 23 Pages: 12223-12232
  • DOI: 10.1074/jbc.m116.719815
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] アニオンチャネルロドプシン２(ACR2)の線虫における超高感度光神経抑制活性 (2017)
  • Author(s): 山梨 太郎, 真木 美紗代, 小島 慧一, 高木 新 、須藤 雄気
  • Organizer: 第55回・生物物理学会