The first attempt at active control of cellular function based on organelle-level thermal handling using nanoparticles

• Project/Area Number: 15K13321
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Nagoya University
• Principal Investigator: Morita Yasuyuki 名古屋大学, 工学研究科, 准教授 (90380534)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ナノ材料 / ナノバイオ / ドラッグデリバリーシステム / 透過膜ペプチド / EPR効果 / がん治療 / 膜透過ペプチド

Outline of Final Research Achievements

A new DDS carrier has been developed for cancer treatment. The nanoparticles are core-shell structure which is Fe3O4 for the core material and SiO2 for the porous shell material. The Fe3O4 is heated by applying alternating magnetic field, and brings out hyperthermia effect. The function of porous SiO2 is for support of anti-cancer drug. Additionally, the surface of the nanoparticles developed were covered with tLyP-1 peptides which home only cancer cells not normal cells, thereby we have succeeded an active targeting DDS for cancer treatment.

Research Products

• [Journal Article] Experimental evaluation of the effect of deposited nanoparticle size on current enhancement of solar cells using the sputtering technique (2016)
  • Author(s): Y. Morita, A. Ishiguro, and Y. Ju
  • Journal Title: Advanced Experimental Mechanics Volume: 1 Pages: 115-119
  • NAID: 130005331725
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cell-induced 3D displacement field of cell-adhesion matrices with differing elasticities (2016)
  • Author(s): Y. Morita, N. Kawase, Y. Ju, and T. Yamauchi
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials Volume: 60 Pages: 394-400
  • Peer Reviewed
• [Journal Article] Development of a new co-culture system, the “separable-close co-culture system,” to enhance stem-cell-to-chondrocyte differentiation (2015)
  • Author(s): Y. Morita, S. Yamamoto, and Y. Ju
  • Journal Title: Biotechnology Letters Volume: 37 Issue: 9 Pages: 1911-1918
  • DOI: 10.1007/s10529-015-1858-5
  • Peer Reviewed
• [Journal Article] In vitro experimental study for the determination of cellular characteristics of mesenchymal stem cells using a non-uniform deformation field (2015)
  • Author(s): Y. Morita, T. Sato, S. Watanabe, and Y. Ju
  • Journal Title: Proceedings of the SEM 2015 Annual Conference and Exposition on Experimental and Applied Mechanics Volume: - Pages: 306-306
  • Peer Reviewed
• [Presentation] Fe3O4@mSiO2-tLyP-1ナノ粒子を用いた標的指向型DDSの開発 (2017)
  • Author(s): 森田康之, 櫻井涼平, 脇本卓磨, 徳悠葵, 巨陽
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウインクあいち
  • Year and Date: 2017-01-19
• [Presentation] コアシェル型ナノ構造体を用いたがんターゲティングDDSの開発 (2016)
  • Author(s): 森田康之, 脇本卓磨, 櫻井涼平, 徳悠葵, 巨陽
  • Organizer: 日本実験力学会 分科会合同ワークショップ2016 －実験力学における計測・データ処理の問題点・ノウハウ・工夫－
  • Place of Presentation: 伊良湖ビューホテル
  • Year and Date: 2016-12-02
• [Presentation] コアシェル型ナノ構造体を用いた機能性DDSの開発に関する研究 (2016)
  • Author(s): 森田康之, 櫻井涼平, 脇本卓磨, 巨陽
  • Organizer: 日本実験力学会 The 15th Conference on Biomechanics in Matsushima
  • Place of Presentation: パレス松洲，松島，宮城
  • Year and Date: 2016-03-19
• [Presentation] 機械的伸縮刺激による腱細胞分化に関する1細胞計測 (2016)
  • Author(s): 山下貴大, 森田康之, 徳悠葵, 巨陽
  • Organizer: 日本機械学会東海支部第65期総会・講演会
  • Place of Presentation: 愛知工業大学，豊田，愛知
  • Year and Date: 2016-03-17
• [Presentation] DVC法による分化時の細胞－細胞外マトリックス間の3D力学場計測 (2016)
  • Author(s): 森田康之, 河瀬直樹, 巨陽, 山内崇司
  • Organizer: 日本機械学会第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学，東京
  • Year and Date: 2016-01-09
• [Presentation] 機械的伸縮刺激における間葉系幹細胞の腱細胞分化に対するひずみ依存性の解明 (2015)
  • Author(s): 森田康之, 佐藤俊博, 巨陽
  • Organizer: 日本機械学会M&M2015材料力学カンファレンス
  • Place of Presentation: 慶應義塾大学，横浜
  • Year and Date: 2015-11-21
• [Presentation] Rigorous optimal cyclic strain for differentiation of mesenchymal stem cells into tenocytes using an exhaustive strain field (2015)
  • Author(s): Y. Morita, T. Sato, and Y. Ju
  • Organizer: International Conference on Advanced Technology in Experimental Mechanics 2015 (ATEM’15) & The 14th Asian Conference on Experimental Mechanics (ACEM14)
  • Place of Presentation: Loisir Hotel Toyohashi, Toyohashi, Aichi
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] 浸潤性がん細胞と非浸潤性がん細胞が細胞外マトリックスに及ぼす変位場の3D計測 (2015)
  • Author(s): 山内崇司, 森田康之, 巨陽, 河瀬直樹
  • Organizer: 日本機械学会第26回バイオフロンティア講演会
  • Place of Presentation: 九州大学，福岡
  • Year and Date: 2015-10-02
• [Presentation] がんのイメージングと温熱治療特性を付加した多機能DDSの創製と評価 (2015)
  • Author(s): 櫻井涼平, 森田康之, 巨陽, 徐百耀
  • Organizer: 日本機械学会2015年度年次大会
  • Place of Presentation: 北海道大学，札幌
  • Year and Date: 2015-09-13
• [Presentation] In vitro experimental study for the determination of cellular characteristics of mesenchymal stem cells using a non-uniform deformation field (2015)
  • Author(s): Y. Morita, T. Sato, S. Watanabe, and Y. Ju
  • Organizer: The SEM 2015 Annual Conference and Exposition on Experimental and Applied Mechanics
  • Place of Presentation: Costa Mesa, California, USA
  • Year and Date: 2015-06-08
  • Int'l Joint Research
• [Presentation] マイクログルーヴ法を用いた間葉系幹細胞の配向制御による腱細胞分化に関する研究 (2015)
  • Author(s): 森田康之, 栗田史哉, 佐藤俊博, 巨陽
  • Organizer: 平成27年度非破壊検査シンポジウム
  • Place of Presentation: 立花アネックスビル，東京都
  • Year and Date: 2015-06-02
• [Book] Experimental and Applied Mechanics (2015)
  • Author(s): Y. Morita, T. Sato, S. Watanabe, and Y. Ju
  • Publisher: Springer