Optical cleaning method of living animal skull for 2photon imaging

• Project/Area Number: 15K13376
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Optical engineering, Photon science
• Research Institution: The University of Tokyo
• Principal Investigator: Onodera Hiroshi 東京大学, 大学院工学系研究科(工学部), 特任教授 (20214207)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 臓器透明化 / 神経科学 / 情報伝達機構 / 透明化技術 / 脳活動リアルタイム観察 / GCAMP / 臓器透明化技術 / 神経生理学 / レーザー顕微鏡

Outline of Final Research Achievements

The aim of this study is to clarify skull bone of mice in order to analyze neuronal network in the cerebral cortex of living animal. First, we successfully optically cleaned mouse skull bone in vitro by using cleaning agent LUCID-vivo. Then we applied the LUCID-vivo solution on living mouse skull bone for 24 hours. We could observe cerebral cortex and blood vessels on the cortex through transparent skull bones. Neuronal networks of the cerebral cortex were visualized by using transgenic mice (GCAMP). This method can be applicable for many research fields, such as memory, motor function, sensory function, and emotion.

Research Products

• [Journal Article] Ultraflexible organic amplifier with biocompatible gel electrodes (2016)
  • Author(s): Tsuyoshi Sekitani, Tomoyuki Yokota, Kazunori Kuribara, Martin Kaltenbrunner, Takanori Fukushima, Yusuke Inoue, Masaki Sekino, Takashi Isoyama, Yusuke Abe Hiroshi, Onodera and Takao Someya
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 11425-11425
  • DOI: 10.1038/ncomms11425
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dendrigraft polylysine coated-poly(glycolic acid) fibrous scaffolds for hippocampal neurons (2016)
  • Author(s): Kojima C, Nishioka E, Imai T, Nakahira A, Onodera H
  • Journal Title: J Biomedical Materials Research Volume: 119 Issue: 33 Pages: 2744-2750
  • DOI: 10.1021/acs.jpcb.5b01553
  • Peer Reviewed / Open Access
• [Journal Article] Optogenetic activation of leptin- and glucoseregulated GABAergic neurons in dorsomedial hypothalamus promotes food intake via inhibitory synaptic transmission to paraventricular nucleus of hypothalamus. (2016)
  • Author(s): Zesemdorj Otgon-Uul , Shigetomo Suyama, Hiroshi Onodera, Toshihiko Yada.
  • Journal Title: Molecular Metabolism Volume: 5 Issue: 8 Pages: 709-715
  • DOI: 10.1016/j.molmet.2016.06.010
  • Peer Reviewed / Open Access
• [Journal Article] Ultraflexible organic amplifier with biocompatible gel electrodes (2016)
  • Author(s): Tsuyoshi Sekitani, Tomoyuki Yokota, Kazunori Kuribara,w, Martin Kaltenbrunner, Takanori Fukushima,Yusuke Inoue, Masaki Sekino, Takashi Isoyama, Yusuke Abe, Hiroshi Onodera, Takao Someya
  • Journal Title: Nature Communications Volume: in press
• [Presentation] In vivoでのマウス 頭蓋骨 透明化 に向けた骨片透明化 (2016)
  • Author(s): 鳴海紘也，小野寺宏
  • Organizer: 応用物理学会秋季学術講演会
  • Place of Presentation: 新潟市
  • Year and Date: 2016-09-13