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Development of Environmental Analysis Methods by High Performance Chromatography Based on Molecular Recognition

Research Project

Project/Area Number 15K13756
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Green/Environmental chemistry
Research InstitutionKyoto University

Principal Investigator

OTSUKA Koji  京都大学, 工学研究科, 教授 (70183762)

Co-Investigator(Kenkyū-buntansha) 久保 拓也  京都大学, 工学研究科, 准教授 (20374994)
内藤 豊裕  京都大学, 工学研究科, 助教 (10711806)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords環境分析 / 分子認識 / 分子インプリント / ナノ炭素材料 / クロマトグラフィー / キャピラリー / フラーレン
Outline of Final Research Achievements

To develop high performance analytical separation techniques for environmental analysis, fundamental studies on highly efficient and selective separation of model compounds were carried out mainly using capillary high performance liquid chromatography (cHPLC). In these studies, novel polymer separation media as well as nano-carbon material immobilized separation media were prepared. The prepared media were then applied to a stationary phase of cHPLC for the environmental analyses, where the possibility of highly selective and high performance separation was successfully demonstrated.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (35 results)

All 2017 2016 2015

All Journal Article (18 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 18 results,  Open Access: 9 results,  Acknowledgement Compliant: 9 results) Presentation (17 results) (of which Int'l Joint Research: 14 results,  Invited: 16 results)

  • [Journal Article] Sensitivity Enhancement by Sweeping <i>via</i> Solid Phase Extraction Using Titania Nanoparticles in Capillary Electrophoretic Analysis of Phosphopeptides2017

    • Author(s)
      Kitagawa, F.; Matsuo, A.; Sueyoshi, K.; Otsuka, K.
    • Journal Title

      CHROMATOGRAPHY

      Volume: 38 Issue: 1 Pages: 39-43

    • DOI

      10.15583/jpchrom.2016.013

    • NAID

      130005439672

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a C<sub>70</sub>-Fullerene Bonded Silica-Monolithic Capillary and Its Retention Characteristics in Liquid Chromatography2017

    • Author(s)
      Kanao, E.; Naito, T.; Kubo, T.; Otsuka, K.
    • Journal Title

      CHROMATOGRAPHY

      Volume: 38 Issue: 2 Pages: 45-51

    • DOI

      10.15583/jpchrom.2016.020

    • NAID

      130007040481

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] New platform for simple and rapid protein-based affinity reactions2017

    • Author(s)
      Kubota, K.; Kubo, T.; Tanigawa, T.; Naito, T.; Otsuka, K.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1

    • DOI

      10.1038/s41598-017-00264-y

    • NAID

      120005997889

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and Characterization of a Thermally Cleaved Fragment of Monoclonal Antibody-A Detected by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis2017

    • Author(s)
      Kubota, K.; Kobayashi, N.; Yabuta, M.; Ohara, M.; Naito, T.; Kubo, T.; Otsuka, K.
    • Journal Title

      J. Pharm. Biomed. Anal.

      Volume: 140 Pages: 98-104

    • DOI

      10.1016/j.jpba.2017.03.027

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional fabrication for microfluidics by conventional techniques and equipment used in mass production2016

    • Author(s)
      Naito, T.; Nakamura, M.; Kaji, N.; Kubo, T.; Baba, Y.; Otsuka, K
    • Journal Title

      Micromachines

      Volume: 7 Issue: 5 Pages: 82-91

    • DOI

      10.3390/mi7050082

    • NAID

      120005825916

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Validation of Capillary Zone Electrophoretic Method for Evaluating Monoclonal Antibodies and Antibody-Drug Conjugates2016

    • Author(s)
      Kubota, K.; Kobayashi, N.; Yabuta, M.; Ohara, M.; Naito, T.; Kubo, T.; Otsuka, K.
    • Journal Title

      CHROMATOGRAPHY

      Volume: 37 Issue: 3 Pages: 117-124

    • DOI

      10.15583/jpchrom.2016.011

    • NAID

      130005277436

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of Acidic Additives on Peak Capacity and Detectivity in Peptide Analysis Using Nano-Flow LC/MS with Low-Density ODS Modified Monolithic Silica Capillary Columns2016

    • Author(s)
      Kobayashi, H.; Sukegawa, M.; Fujimura, K.; Kubo, T.; Otsuka, K.
    • Journal Title

      CHROMATOGRAPHY

      Volume: 37 Issue: 3 Pages: 133-139

    • DOI

      10.15583/jpchrom.2016.012

    • NAID

      130005277437

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Specific Intermolecular Interactions by the Localized p-Electrons in C70-fullerene2016

    • Author(s)
      Kubo, T.; Kanao, E.; Matsumoto, T.; Naito, T.; Sano, T.; Yan, M.; Otsuka, K.
    • Journal Title

      ChemistrySelect

      Volume: 1 Issue: 18 Pages: 5900-5904

    • DOI

      10.1002/slct.201601470

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] On-line coupling of sample preconcentration by LVSEP with gel electrophoretic separation on T-channel chips2016

    • Author(s)
      F. Kitagawa, S. Kinami, Y. Takegawa, I. Nukatsuka, K. Sueyoshi, T. Kawai, K. Otsuka
    • Journal Title

      Electrophoresis

      Volume: 38 Issue: 2 Pages: 380-386

    • DOI

      10.1002/elps.201600184

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent progress for the selective pharmaceutical analyses using molecularly imprinted adsorbents and their related techniques: A review2016

    • Author(s)
      Kubo, T.; Otsuka, K.
    • Journal Title

      J. Pharm. Biomed. Anal.

      Volume: 130 Pages: 68-80

    • DOI

      10.1016/j.jpba.2016.05.044

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Selective adsorption of trypsin using molecularly imprinted polymers prepared with PEG-based hydrogels containing anionic functional monomers2016

    • Author(s)
      Kubo, T.; Arimura, S.; Naito, T.; Otsuka, K.
    • Journal Title

      Mol. Imprinting

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Recent progress in molecularly imprinted media by new preparation concepts and methodological approaches for selective separations of targeting compounds2016

    • Author(s)
      Kubo, T.; Otsuka, K.
    • Journal Title

      Trends Anal. Chem.

      Volume: 81 Pages: 102-109

    • DOI

      10.1016/j.trac.2015.08.008

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Simple and Rapid Immobilization of Coating Polymers on Poly(dimethyl siloxane)-glass Hybrid Microchips by a Vacuum-drying Method2015

    • Author(s)
      Kitagawa, F.; Nakagawara, S.; Nukatsuka, I.; Hori, Y.; Sueyoshi, K.; Otsuka, K.
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 11 Pages: 1171-1175

    • DOI

      10.2116/analsci.31.1171

    • NAID

      130005108598

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Unique Separation Behavior of C60-fullerene Bonded Silica Monolith Prepared via an Effective Thermal Coupling Agent2015

    • Author(s)
      Kubo, T.; Murakami, Y.; Tsuzuki, M.; Kobayashi, H.; Naito, T.; Sano, T.; Yan, M.; Otsuka, K.
    • Journal Title

      Chem. Eur. J.

      Volume: 21 Issue: 50 Pages: 18095-18098

    • DOI

      10.1002/chem.201503898

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] C<sub>60</sub>-Fullerene Bonded Silica Monolithic Capillary for Specific Separations of Aromatic Compounds2015

    • Author(s)
      Kubo, T.; Murakami, Y.; Naito, T.; Otsuka, K.
    • Journal Title

      CHROMATOGRAPHY

      Volume: 36 Issue: 3 Pages: 105-113

    • DOI

      10.15583/jpchrom.2015.032

    • NAID

      130005118198

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Molecularly Imprinted Polymers for Selective Adsorption of Lysozyme and Cytochrome c Using a PEG-Based Hydrogel: Selective Recognition for Different Conformations due to pH Conditions2015

    • Author(s)
      Kubo, T.; Arimura, S.; Tominaga, Y.; Naito, T.; Hosoya, K.; Otsuka, K.
    • Journal Title

      Macromolecules

      Volume: 48 Issue: 12 Pages: 4081-4087

    • DOI

      10.1021/acs.macromol.5b00834

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecularly Imprinted Polymer with a Pseudo-template for Thermo-responsive Adsorption/desorption based on Hydrogen Bonding2015

    • Author(s)
      Kubo, T.; Koterasawa, K.; Naito, T.; Otsuka, K.
    • Journal Title

      Micropor. Mesopor. Mater.

      Volume: 218 Pages: 112-117

    • DOI

      10.1016/j.micromeso.2015.07.006

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Simple Preparation and Characterization of Viscoelastic Gels Induced by Multiple Intermolecular Interactions Using Low Molecular Weight Species2015

    • Author(s)
      Kubo, T.; Kuroda, K.; Naito, T.; Mukai, S.; Sano, T.; Akiyoshi, K.; Otsuka, K.
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 88 Issue: 11 Pages: 1575-1580

    • DOI

      10.1246/bcsj.20150209

    • NAID

      130005108566

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Strategies for Microscale Liquid Phase Separations of Biogenic Compounds2016

    • Author(s)
      Kubo, T.; Naito, T.; Otsuka, K.
    • Organizer
      27th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2016)
    • Place of Presentation
      Guangzhou (China)
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microscale Liquid Phase Specific Separations Using Fullerenes2016

    • Author(s)
      Kubo, T.; Kanao, E.; Naito, T.; Otsuka, K.
    • Organizer
      16th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2016)
    • Place of Presentation
      Johor Bahru, Johor (Malaysia)
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 特異的相互作用を利用する分離分析2016

    • Author(s)
      大塚浩二
    • Organizer
      第47回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      豊橋技術科学大学, 豊橋
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Microscale Specific Separations Using Fullerenes2016

    • Author(s)
      Otsuka, K.; Tsuzuki, M.; Kanao, E.; Naito, T.; Kubo, T
    • Organizer
      23rd International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP 2016),
    • Place of Presentation
      Minneapolis, MN (USA)
    • Year and Date
      2016-09-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 特異的相互作用を利用する分離場の開発2016

    • Author(s)
      久保拓也, 内藤豊裕, 大塚浩二
    • Organizer
      第29回バイオメディカル分析科学シンポジウム (BMAS2016)
    • Place of Presentation
      京都大学・桂キャンパス, 京都
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Specific Retention Behaviors of a C70-fullerene Bonded Silica-monolithic Capillary in Liquid Chromatography2016

    • Author(s)
      Kubo, T.; Kanao, E.; Kobayashi, H.; Naito, T.; Yan, M.; Otsuka, K.
    • Organizer
      44th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2016 San Francisco)
    • Place of Presentation
      San Francisco, CA (USA)
    • Year and Date
      2016-06-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High Performance Microscale Liquid Phase Separations of Biogenic Compounds2016

    • Author(s)
      Naito, T.; Kubo, T.; Otsuka, K.
    • Organizer
      44th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2016 San Francisco)
    • Place of Presentation
      San Francisco, CA (USA)
    • Year and Date
      2016-06-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] High performance microscale separations using specific interactions2016

    • Author(s)
      Kubo, T.; Tsuzuki, M.; Kanao, E.; Naito, T.; Otsuka, K.
    • Organizer
      40th International Symposium on Capillary Chromatography (ISCC2016),
    • Place of Presentation
      Riva del Garda (Italy)
    • Year and Date
      2016-05-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 近未来における分離科学の新展開2016

    • Author(s)
      大塚浩二
    • Organizer
      日本分析化学会第76回分析化学討論会
    • Place of Presentation
      岐阜薬科大学・岐阜大学(岐阜県・岐阜市)
    • Year and Date
      2016-05-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] High performance microscale liquid phase separations of biomolecules2015

    • Author(s)
      Fukushima, Y.; Naito, T.; Kubo, T.; Otsuka, K.
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Honolulu, HI (USA)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] MEKC in 30 Years2015

    • Author(s)
      Otsuka, K.
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Honolulu, HI (USA)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The use of C60-fullerene bonded silica monolith as a molecular recognition medium for microscale separation2015

    • Author(s)
      Kubo, T.; Tsuzuki, M.; Murakami, Y.; Naito, T.; Otsuka, K.
    • Organizer
      15th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2015)
    • Place of Presentation
      Tainan (Taiwan, ROC)
    • Year and Date
      2015-11-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of novel separation media for microscale separation2015

    • Author(s)
      Fukushima, Y.; Naito, T.; Kubo, T.; Otsuka, K.
    • Organizer
      43rd International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2015 Beijing)
    • Place of Presentation
      Beijing (China)
    • Year and Date
      2015-09-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of nano carbon-material bonded silica monolith as separation media for microscale LC and CE2015

    • Author(s)
      Murakami, Y.; Tsuzuki, M.; Naito, T.; Kubo, T.; Otsuka, K.
    • Organizer
      10th Balaton Symposium on High-Performance Separation Methods
    • Place of Presentation
      Siofok (Hungary)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of silica monolith as molecular recognition media for microscale separation2015

    • Author(s)
      Kubo, T.; Naito, T.; Otsuka, K.
    • Organizer
      22nd International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015)
    • Place of Presentation
      Helsinki (Finland)
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of a molecularly imprinted PEG-based hydrogel for protein recognition2015

    • Author(s)
      Otsuka, K.; Kubo, T.; Arimura, S.; Naito, T.
    • Organizer
      26th International Symposium on Pharmaceutical and Biomedical Analysis (PBA2015)),
    • Place of Presentation
      Tbilisi (Georgia)
    • Year and Date
      2015-07-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Simplified high performance CE separations of biomolecules2015

    • Author(s)
      Fukushima, Y.; Naito, T.; Kubo, T.; Otsuka, K.
    • Organizer
      42nd International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2015 Geneva)
    • Place of Presentation
      Geneva (Switzerland)
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2018-03-22  

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