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High-through culture method enabling the genome-phenome integration screening system

Research Project

Project/Area Number 15K14231
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionNagaoka University of Technology

Principal Investigator

Ogasawara Wataru  長岡技術科学大学, 工学研究科, 教授 (40292172)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords微生物スクリーニング / エマルション / 培養 / スクリーニング / 油脂生産酵母 / 環境微生物 / 1細胞分離
Outline of Final Research Achievements

The ultimate goal of this study is the development of a next generation screening system "genome-phenome integration screening system" that integrates genome analysis and comprehensive phenotypic analysis. For this study, one cell separation, cultivation and screening system are essential. Therefore, we attempted "establishment of a cell screening system for eukaryotic microorganisms" relatively easy . We succeeded in screening of higher lipid production oleaginous yeast strain. Next, we attempted "establishment of screening system for eubacteria" relatively difficult. We succeeded in cultivation and screening of model organisms, cultivation of environmental microorganisms and microbial flora analysis.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (17 results) (of which Int'l Joint Research: 4 results) Remarks (2 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] Crystal structures of a bacterial dipeptidyl peptidase IV reveal a novel substrate recognition mechanism distinct from that of mammalian orthologues2018

    • Author(s)
      Roppongi Saori、Suzuki Yoshiyuki、Tateoka Chika、Fujimoto Mayu、Morisawa Saori、Iizuka Ippei、Nakamura Akihiro、Honma Nobuyuki、Shida Yosuke、Ogasawara Wataru、Tanaka Nobutada、Sakamoto Yasumitsu、Nonaka Takamasa
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 2714-2714

    • DOI

      10.1038/s41598-018-21056-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Periplasmic form of dipeptidyl aminopeptidase IV from Pseudoxanthomonas mexicana WO24: purification, kinetic characterization, crystallization and X‐ray crystallographic analysis2017

    • Author(s)
      Saori Roppongi Chika Tateoka Mayu Fujimoto Ippei Iizuka Saori Morisawa Akihiro Nakamura Nobuyuki Honma Yoshiyuki Suzuki Yosuke Shida Wataru Ogasawara Nobutada Tanaka Yasumitsu Sakamoto Takamasa Nonaka
    • Journal Title

      Acta Crystallographica Section F

      Volume: 73 Issue: 11 Pages: 501-606

    • DOI

      10.1107/s2053230x17014911

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Pseudoxanthomonas mexicana WO24由来Dipeptidyl aminopeptidase IVの基質認識機構の解明2018

    • Author(s)
      中村彰宏, 本間宣行, 六本木沙織, 鈴木義之, 志田洋介, 阪本泰光, 飯塚一平, 伊中浩治, 田仲広明, 木平清人 ,山田貢, 吉崎泉, 合田浩 明, 野中孝昌, 田中信忠, 小笠原渉
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 部位特異的変異導入解析によるFamily S46ペプチダーゼの基質認識残基の同定2018

    • Author(s)
      本間宣行, 中村彰宏, 六本木沙織, 鈴木義之, 志田洋介, 阪本泰光, 飯塚一平, 伊中浩治, 田仲広明, 木平清人, 山田貢, 吉崎泉, 合田浩明, 野中孝昌, 田中信忠, 小笠原渉
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Biochemical and Structural Analysis of Bacterial Distinctive Peptidase2017

    • Author(s)
      Akihiro Nakamura, Yasuhiro Ito, Yoshiyuki Suzuki), Yasumitsu Sakamoto, Nobutada Tanaka, Wataru Ogasawara
    • Organizer
      地域活性に関する国際会議(ISLife2017)
    • Place of Presentation
      長島町文化ホール,サンセット長島
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of Substrate Recognition Residues in Family S46 Peptidase from Pathogenic Non-Fermenting Gram- Negative Bacteria2017

    • Author(s)
      Akihiro Nakamura, Yasuhiro Ito, Yoshiyuki Suzuki, Saori Roppogi, Koushi Hidaka, Yasumitsu Sakamoto, Nobutada Tanaka, Wataru Ogasawara
    • Organizer
      IUMS2017 SINGAPORE
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of Substrate Recognition Residues in Family S46 Peptidase from Gram-Negative Bacteria2017

    • Author(s)
      Akihiro Nakamura, Yoshiyuki Suzuki, Saori Roppongi, Koushi Hidaka, Yasumitsu Sakamoto, Nobutada Tanaka, Wataru Ogasawara
    • Organizer
      The 6th International GIGAKU Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糸状菌 Trichoderma reesei における分泌プロテアーゼの生産応答機構の解析2017

    • Author(s)
      鈴木義之, Nayani Daranagama, 志田洋介, 森一樹, 油谷幸代, 小笠原渉
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Annual Research Report
  • [Presentation] WO24由来Dipeptidyl aminopeptidase IVの構造2017

    • Author(s)
      中村 彰宏, 本間 宣行, 六本木 沙織, 鈴木 義之, 志田 洋介, 阪本 泰光, 飯塚 一平, 伊中 浩治, 田中 広明, 木平 清人, 山 田 貢, 吉崎 泉
    • Organizer
      第12回日本ゲノム微生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Enzymatic characterization of S46 peptidase from pathogenic bacteria2016

    • Author(s)
      Yasuhiro Ito, Akihiro Nakamura, Saori Roppongi, Yuki Sakurai, Yosiyuki Suzuki, Koushi Hidaka, Yuko Tsuda, Yasumitsu Sakamoto, Nobutada Tanaka, Wataru Ogasawara
    • Organizer
      The 5th International GIGAKU Conference in Nagaoka (IGCN2016)
    • Place of Presentation
      長岡技術科学大学
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 病原菌由来S46ペプチダーゼの酵素学的諸性質の解明2016

    • Author(s)
      伊藤 康広, 鈴木 義之, 志田 洋介, 阪本 泰光, 田中 信忠, 小笠原 渉
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場,ANAクラウンプラザホテル富山
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 病原菌由来S46ペプチダーゼにおける構造機能相関の解明2016

    • Author(s)
      伊藤 康広, 鈴木 義之, 阪本 泰光, 日高 興士, 田中 信忠, 小笠原 渉
    • Organizer
      第1回高専生サミット on Bioinspired Chemistry
    • Place of Presentation
      鶴岡工業高等専門学校K-ARC
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
  • [Presentation] Porphyromonas endodontalis由来 dipeptidyl peptidese 11 の精製と機能解析 Expression, purification and characterization of dipeptidyl peptidese 11 from the periodontal pathogen Porphyromonas endodontalis2016

    • Author(s)
      中村 彰宏, 伊藤 康広, 鈴木 義之, 志田 洋介, 阪本 泰光, 田中 信忠, 小笠原 渉
    • Organizer
      第16回蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
  • [Presentation] S46ペプチダーゼの構造機能相関の解明  Elucidation of structure function relationship in family S46 peptidases2016

    • Author(s)
      伊藤 康広, 鈴木 義之, 阪本 泰光, 田中 信忠, 小笠原 渉
    • Organizer
      第16回蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 油脂生産酵母 Rhodosporium toruloides の突然変異導入による変異遺伝子の同定2016

    • Author(s)
      宮田淳史, 通野和人, 高見沢健留, 志田洋介, 山崎晴丈, 正木和夫, 森 一樹, 久原 哲, 高久洋暁,小笠原 渉
    • Organizer
      第10回 日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大学 大岡山キャンパス(目黒区)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂酵母Lipomyces starkeyiの油脂高生産変異株の解析2016

    • Author(s)
      高見沢健留, 宮田淳史, 通野和人, 志田洋介, 山崎晴丈, 正木和夫, 森 一樹, 久原 哲, 高久洋暁, 小笠原 渉
    • Organizer
      第10回 日本ゲノム微生物学会年会
    • Place of Presentation
      第10回 日本ゲノム微生物学会年会
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂生産酵母Rhodosporidium toruloidesの光応答によるカロテノイド生合成機構の解明2016

    • Author(s)
      宮田 淳史, 志田 洋介, 山崎 晴丈, 正木 和夫,森 一樹, 久原 哲, 高久 洋暁, 小笠原 渉
    • Organizer
      第11回 バイオマス科学会議
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟市)
    • Year and Date
      2016-01-20
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂生産酵母Rhodosporidium toruloidesの突然変異導入による油脂生産関連遺伝子の同定2015

    • Author(s)
      宮田淳史, 通野和人, 志田洋介, 山崎晴丈, 正木和夫, 森 一樹, 久原 哲, 高久洋暁, 小笠原 渉
    • Organizer
      第67回 日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 油脂酵母Lipomyces starkeyiの油脂生産に重要な遺伝子の探索2015

    • Author(s)
      通野和人, 宮田淳史, 志田洋介, 山崎晴丈, 森 一樹, 久原 哲, 高久洋暁, 小笠原 渉
    • Organizer
      第67回 日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Remarks] 長岡技術科学大学小笠原研究室

    • URL

      http://www.microorganisms.jp/ogasawara-lab/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 発酵を科学する まだ見ぬ‘美’生物の世界~99%へのプロローグ~

    • URL

      http://www.microorganisms.jp/tp://www.microorganisms.jp/

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] ペプチド型細菌ジペプチジルペプチダーゼ7阻害剤2017

    • Inventor(s)
      柴田 望、小笠原 渉、志田 洋介
    • Industrial Property Rights Holder
      柴田 望、小笠原 渉、志田 洋介
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 改変プロモーター2017

    • Inventor(s)
      掛下 大視,柴田 望、小笠原 渉、志 田 洋介
    • Industrial Property Rights Holder
      掛下 大視,柴田 望、小笠原 渉、志 田 洋介
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 糸状菌変異株及びその利用2017

    • Inventor(s)
      柴田 望、小笠原 渉、志田 洋介
    • Industrial Property Rights Holder
      柴田 望、小笠原 渉、志田 洋介
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 糸状菌変異株及びその利用2016

    • Inventor(s)
      小笠原渉,志田洋介,柴田望
    • Industrial Property Rights Holder
      小笠原渉,志田洋介,柴田望
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-08-02
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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