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Development of genetically encoded protein synthesis activity reporter

Research Project

Project/Area Number 15K14311
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionThe University of Tokyo

Principal Investigator

Ikeuchi Yoshiho  東京大学, 生産技術研究所, 講師 (30740097)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsタンパク質合成 / 翻訳 / リボソーム / 神経 / 形態 / レポーター / GFP
Outline of Final Research Achievements

In this study, we developed a reporter protein for cellular protein synthesis that is altered upon neural plasticity. To directly represent the cellular protein synthesis activity at the time of measurement, we designed an artificial reporter protein that is only fluorescent during it is under synthesis, and lose fluorescence after protein synthesis by self digestion. We generated the construct, expressed the proteins, and checked the self digestion ability of the reporter. We optimized the construct to maximize the self digestion ability.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (1 results)

All 2017

All Journal Article (1 results) (of which Acknowledgement Compliant: 1 results)

  • [Journal Article] New approaches to understand dynamics of protein synthesis in neurons2017

    • Author(s)
      Angela Chow, Yoshiho Ikeuchi
    • Journal Title

      生産研究

      Volume: 69 Pages: 31-36

    • NAID

      130005682802

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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