Development of genetically encoded protein synthesis activity reporter

Research Project

Project/Area Number	15K14311
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Neurophysiology / General neuroscience
Research Institution	The University of Tokyo
Principal Investigator	Ikeuchi Yoshiho 東京大学, 生産技術研究所, 講師 (30740097)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	タンパク質合成 / 翻訳 / リボソーム / 神経 / 形態 / レポーター / GFP
Outline of Final Research Achievements	In this study, we developed a reporter protein for cellular protein synthesis that is altered upon neural plasticity. To directly represent the cellular protein synthesis activity at the time of measurement, we designed an artificial reporter protein that is only fluorescent during it is under synthesis, and lose fluorescence after protein synthesis by self digestion. We generated the construct, expressed the proteins, and checked the self digestion ability of the reporter. We optimized the construct to maximize the self digestion ability.