| Project/Area Number |
15K14311
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | タンパク質合成 / 翻訳 / リボソーム / 神経 / 形態 / レポーター / GFP |
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| Outline of Final Research Achievements |
In this study, we developed a reporter protein for cellular protein synthesis that is altered upon neural plasticity. To directly represent the cellular protein synthesis activity at the time of measurement, we designed an artificial reporter protein that is only fluorescent during it is under synthesis, and lose fluorescence after protein synthesis by self digestion. We generated the construct, expressed the proteins, and checked the self digestion ability of the reporter. We optimized the construct to maximize the self digestion ability.
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