Exploring the mechanical force driving nuclear migration in developing neurons
| Project/Area Number |
15K14317
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Kyoto University |
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Principal Investigator |
KENGAKU Mineko 京都大学, 物質－細胞統合システム拠点, 教授 (10303801)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEKO Makoto 大阪大学, 大学院工学研究科, 教授 (70224607)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 神経科学 / 発生・分化 / 細胞・組織 / ライブイメージング |
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| Outline of Final Research Achievements |
Neuronal migration is a critical step for cortical formation in the mammalian brain, yet the precise mechanism of cytoskeletal force transmission is not fully understood. Using high resolution confocal imaging, we visualized dynamic deformation and rotation of the nucleus in migrating neurons. We demonstrated that microtubules dynamically bind to small points on the nuclear envelope via the plus- and minus-end oriented motors, kinesin and dynein, and induce sharpening, rotation and translocation of the nucleus via LINC complex.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Mitochondrial fission protein Drp1 regulates mitochondrial transport and dendritic arborization in cerebellar Purkinje cells.2016
Author(s)
Fukumitsu, K., Hatsukano, T., Yoshimura, A., Heuser, J., Fujishima, K. and Kengaku, M.
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Journal Title
Mol Cell Neurosci.
Volume: 71
Pages: 56-65
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.2015
Author(s)
Oomoto, I., Suzuki-Hirano, A., Umeshima, H., Han, YW., Yanagisawa, H., Carlton, P., Harada, Y., Kengaku, M., Okamoto, A., Shimogori, T. and Wang, DO.
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Journal Title
Nucleic Acids Res.
Volume: 43(19)
Issue: 19
Pages: e126-e126
DOI
NAID
Related Report
Peer Reviewed / Open Access
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